CAJ | 학술논문

目的：观察健脾补肾方促进骨髓间充质干细胞（Bone mesenchymal stem cells，BMSCs）增殖、分化治疗溃疡性结肠炎（Ulcerative colitis，UC）模型大鼠的可能作用机制。方法 SD 大鼠随机分为空白组、模型组、BMSCs 组、干预 BMSCs 组和联合组。采用 TNBS 法建立大鼠 UC 模型，干预 BMSCs 和联合组大鼠分别尾静脉注射体外中药干预的 BMSCs（0．78 mg／L，1×106），联合组再予健脾补肾方（浓度13．6 g／kg）灌胃10 d，BMSCs 组大鼠尾静脉注射1 mL BMSCs（1×106），空白组、模型组大鼠尾静脉分别注射1 mL 生理盐水。干预第10天每组各处死5只大鼠，留取标本，Western blot 法检测大鼠结肠组织肠干细胞标记物 Lgr5和 Ephrin-B3的蛋白表达水平，检测 E-cadherin 的蛋白表达水平，采用 ELISA 法检测各组大鼠结肠组织 IL-6、IL-17和 TGF-β水平。结果各移植治疗组较模型组，Lgr5和 Ephrin-B3蛋白水平升高（P ＜0．05），联合组 Lgr5和 Ephrin-B3蛋白表达较 BMSCs 组，差异有统计学意义（P ＜0．05）；各治疗组 E-cadherin 蛋白表达较模型组均升高，其中联合组和干预BMSCs 组较模型组，差异有统计学意义（P ＜0．05），联合组较 BMSCs 组，差异有统计学意义（P ＜0．05）；联合组和干预 BMSCs组 IL-6、IL-17水平明显降低（P ＜0．05），TGF-β水平升高（P ＜0．05），且联合组在改善 IL-6、TGF-β水平方面较单纯 BMSCs 治疗有统计学意义（P ＜0．05）。结论健脾补肾方能够促进静脉移植的 BMSCs 向肠干细胞增殖分化，并调节免疫功能、修复肠黏膜，这可能是其治疗 UC 的可能机制之一。
목적：관찰건비보신방촉진골수간충질간세포（Bone mesenchymal stem cells，BMSCs）증식、분화치료궤양성결장염（Ulcerative colitis，UC）모형대서적가능작용궤제。방법 SD 대서수궤분위공백조、모형조、BMSCs 조、간예 BMSCs 조화연합조。채용 TNBS 법건립대서 UC 모형，간예 BMSCs 화연합조대서분별미정맥주사체외중약간예적 BMSCs（0．78 mg／L，1×106），연합조재여건비보신방（농도13．6 g／kg）관위10 d，BMSCs 조대서미정맥주사1 mL BMSCs（1×106），공백조、모형조대서미정맥분별주사1 mL 생리염수。간예제10천매조각처사5지대서，류취표본，Western blot 법검측대서결장조직장간세포표기물 Lgr5화 Ephrin-B3적단백표체수평，검측 E-cadherin 적단백표체수평，채용 ELISA 법검측각조대서결장조직 IL-6、IL-17화 TGF-β수평。결과각이식치료조교모형조，Lgr5화 Ephrin-B3단백수평승고（P ＜0．05），연합조 Lgr5화 Ephrin-B3단백표체교 BMSCs 조，차이유통계학의의（P ＜0．05）；각치료조 E-cadherin 단백표체교모형조균승고，기중연합조화간예BMSCs 조교모형조，차이유통계학의의（P ＜0．05），연합조교 BMSCs 조，차이유통계학의의（P ＜0．05）；연합조화간예 BMSCs조 IL-6、IL-17수평명현강저（P ＜0．05），TGF-β수평승고（P ＜0．05），차연합조재개선 IL-6、TGF-β수평방면교단순 BMSCs 치료유통계학의의（P ＜0．05）。결론건비보신방능구촉진정맥이식적 BMSCs 향장간세포증식분화，병조절면역공능、수복장점막，저가능시기치료 UC 적가능궤제지일。
OBJECTIVE To explore the mechanism of ulcerative colitis(UC)treatment by Jianpi-Bushen Fang through pro-moting the differentiation of BMSCs in model rats.METHODS The rats were divided into 5 groups:normal group,model group,BMSCs group,BMSCs intervene group and combine group.BMSCs intervene group and combine group were injected with decoction intervened BMSCs through tail veins in vitro (1×106/mL),the combine group were also given the decoction in oral for 10 d.BMSCs group were injected with BMSCs through tail veins(1×10 6/mL).The normal and model groups were in-jected with 1 mL of normal saline through tail veins.5 rats were killed on 10th day after the intervention,respectively,for specimen.The expression of Lgr5,Ephrin-B3 and E-cadherin were detected with Western blot,and the levels of IL-6,IL-17 and TGF-βwith ELISA assay.RESULTS The expression of Lgr5 and Ephrin-B3 in treatment groups increased(P <0.05), compared with the model group.E-cadherin in treatment groups increased compared with the model group,and the combine group and BMSCs intervene group increased significantly(P <0.05).The levels of IL-6 and IL-17 in combine group and BM-SCs group significantly decreased(P <0.05),while TGF-βincreased(P <0.05).The difference between the combine group and BMSCs group was significant(P <0.05) in Lgr5,Ephrin-B3,E-cadherin,IL-6 and TGF-βexpression.CONCLUSION Jianpi-Bushen Fang can promote the venously transplanted BMSCs proliferation and differentiation into intestinal stem cell, regulate immune function and repair intestinal mucosa,which may be one possible mechanism for UC treatment.