CAJ | 학술논문

目的：从离体肠组织通透性的角度，探讨中药“十八反”中“藻戟遂芫”与甘草合用产生禁忌的可能机理。方法以FITC-右旋糖苷4 kDa （FD4）及 FITC-右旋糖苷40 kDa （FD40）为指示剂，采用 Ussing chamber 技术分别观察“藻戟遂芫”与甘草合用对大鼠离体回肠组织旁路途径通透性及屏障功能的影响。结果甘草与芫花明显提升回肠组织 FD4通透量，大戟和甘遂对 FD4透过量影响不显著；芫花、海藻、大戟、甘遂与甘草合用后，FD4透过量与甘草组相比均显著降低。甘草、海藻、甘遂对 FD40透过量没有显著影响；大戟和芫花单用能显著增加 FD40透过量，二者与甘草合用后 FD40透过量不变或略下降；海藻、甘遂与甘草合用后 FD40透过量与单用组比显著上升。结论“藻戟遂芫”与甘草合用阻碍了甘草增加肠道通透性的效应，可能是其与甘草“相反”的机制之一。另一方面，大戟和芫花对于离体肠组织屏障功能可产生明显损伤，合用甘草之后有一定改善，体现了甘草的黏膜保护作用；海藻和甘遂单用对肠组织屏障功能影响不明显，而与甘草合用后肠道屏障功能受到明显损伤。“藻戟遂芫”和甘草合用后肠道旁路转运能力及屏障功能的改变可能导致在特定条件下产生减效或增毒等“相反”的效应。
목적：종리체장조직통투성적각도，탐토중약“십팔반”중“조극수원”여감초합용산생금기적가능궤리。방법이FITC-우선당감4 kDa （FD4）급 FITC-우선당감40 kDa （FD40）위지시제，채용 Ussing chamber 기술분별관찰“조극수원”여감초합용대대서리체회장조직방로도경통투성급병장공능적영향。결과감초여원화명현제승회장조직 FD4통투량，대극화감수대 FD4투과량영향불현저；원화、해조、대극、감수여감초합용후，FD4투과량여감초조상비균현저강저。감초、해조、감수대 FD40투과량몰유현저영향；대극화원화단용능현저증가 FD40투과량，이자여감초합용후 FD40투과량불변혹략하강；해조、감수여감초합용후 FD40투과량여단용조비현저상승。결론“조극수원”여감초합용조애료감초증가장도통투성적효응，가능시기여감초“상반”적궤제지일。령일방면，대극화원화대우리체장조직병장공능가산생명현손상，합용감초지후유일정개선，체현료감초적점막보호작용；해조화감수단용대장조직병장공능영향불명현，이여감초합용후장도병장공능수도명현손상。“조극수원”화감초합용후장도방로전운능력급병장공능적개변가능도치재특정조건하산생감효혹증독등“상반”적효응。
OBJECTIVE To explore the mechanism of glycyrrhizae's incompatibility against″zao-ji-sui-yuan″(Sargassum, Euphorbia pekinensis,Euphorbia kansui and Daphne genkwa) in TCM ″Shibafan″,from the perspective of intestinal tissue permeability in vitro .METHODS Ussing chamber technique was used to observe the effect of Sargassum,Euphorbia pekin-ensis,Euphorbia kansui and Daphne genkwa,combined with glycyrrhizae on ileum tissue permeability in rats in vitro ,with FITC-dextran 4 kDa(FD4) and FITC-dextran 40 kDa(FD40)as indicators.RESULTS Glycyrrhizae and Daphne genkwa sig-nificantly increased the permeability of FD4 in ileum tissue,while Euphorbia pekinensis and Euphorbia kansui showed little im-pact.Compared with glycyrrhizae group,the FD4 permeability significantly decreased after Sargassum,Euphorbia pekinensis, Euphorbia kansui and Daphne genkwa combined with glycyrrhizae.Remarkable effects were not found in glycyrrhizae,Sargas-sum and Euphorbia kansui groups on FD40 permeability,while Euphorbia pekinensis and Daphne genkwa groups significantly increased.FD40 permeability was found unchanged or slightly decreased after glycyrrhizae was combined with Euphorbia pe-kinensis and Daphne genkwa,while FD40 permeability significantly increased after glycyrrhizae was combined with Sargassum and Euphorbia kansui.CONCLUSION ″Zao-ji-sui-yuan″ hinders the improvement effect of glycyrrhizae on intestinal permea-bility,which is likely to be one of the mechanisms of incompatibility.On the other hand,Euphorbia pekinensis and Daphne genkwa can damage in vitro intestinal barrier function obviously,but not improve significantly after combined with glycyrrhi-zae.Sargassum and Euphorbia kansui effect little on in vitro intestinal barrier function,but damage obviously after combined with glycyrrhizae.Above all,the changes of intestinal bypass transport capacity and barrier function after combining″zao-ji-sui-yuan″ with glycyrrhizae,possibly lead to toxicity or decreased effect in certain conditions.