CAJ | 학술논문

目的：研究重金属 Hg 对黑三棱 Sparganium stoloniferum Buch．-Ham 生长特性及超微结构的影响。方法分别采用LI-6400光合仪及 CCM-200型叶绿素仪对黑三棱的光合、蒸腾速率和叶绿素含量（CCI 值）进行测定，并通过 H7650型透射电镜对其叶片超微结构进行观察。结果在 Hg2＋处理下，随着 Hg2＋处理浓度的增加及污染时间的延长，三棱叶褪绿变黄，药用部位块茎鲜质量明显降低，CCI 值、净光合速率（Pn ）及蒸腾速率（Tr ）下降，细胞间隙 CO2浓度（Ci ）增大，叶肉细胞中细胞核核仁分散，核膜破损，叶绿体膨胀至解体，线粒体变形，嵴突膨胀、减少。结论重金属 Hg 污染破坏三棱叶细胞结构，影响其光合作用，使其生长发育受阻，产量减少，品质降低。
목적：연구중금속 Hg 대흑삼릉 Sparganium stoloniferum Buch．-Ham 생장특성급초미결구적영향。방법분별채용LI-6400광합의급 CCM-200형협록소의대흑삼릉적광합、증등속솔화협록소함량（CCI 치）진행측정，병통과 H7650형투사전경대기협편초미결구진행관찰。결과재 Hg2＋처리하，수착 Hg2＋처리농도적증가급오염시간적연장，삼릉협퇴록변황，약용부위괴경선질량명현강저，CCI 치、정광합속솔（Pn ）급증등속솔（Tr ）하강，세포간극 CO2농도（Ci ）증대，협육세포중세포핵핵인분산，핵막파손，협록체팽창지해체，선립체변형，척돌팽창、감소。결론중금속 Hg 오염파배삼릉협세포결구，영향기광합작용，사기생장발육수조，산량감소，품질강저。
OBJECTIVE To study the effects of Hg on growth characteristics and ultrastructure of Sparganium stolonife-rum .METHODS The photosynthetic characteristics and transpiration rate were analyzed by LI-6400 Portable Photosynthesis System while the chlorophyll was measured by CCM-200 plus Chlorophyll Content Meter.Furthermore we also used H7650 transmission electron microscope to observe the ultrastructure of the blades.RESULTS The results showed that with the ris-ing concentration of Hg2+ and the increasing polluted time,Pn ,Tr and chlorophyll content decreased except Ci ,and fresh weight of medicinal parts tuber also decreased.Meanwhile,the nuclear membrane was broken and nucleoli disappeared.The chloroplast swelled to collapse and the cristae of mitochondria swelled and decreased.CONCLUSION The study suggested that the heavy metal Hg can lead to the destruction of the ultrastructure and photosynthetic of S.stoloniferum and it also affected its production and quality.