化学传感器
化學傳感器
화학전감기
Chemical Sensors
2015年
3期
59-65
,共7页
免疫传感器%甲胎蛋白%二氧化硅%壳聚糖%纳米复合物
免疫傳感器%甲胎蛋白%二氧化硅%殼聚糖%納米複閤物
면역전감기%갑태단백%이양화규%각취당%납미복합물
immunosensor%AFP%SiO2%chitosan%nano composite
首先制备二氧化硅(SiO2)-壳聚糖(chitosan,Cs)纳米复合物。将其修饰在玻碳电极表面,利用纳米SiO2高的表面能和多孔结构吸附电子媒介体硫堇(Thi)。通过Thi中的氨基吸附纳米金(nano-Au),从而固载甲胎蛋白抗体(anti-AFP),并用牛血清白蛋白(BSA)封闭非特异性吸附位点,成功制得一种新型的甲胎蛋白免疫传感器。在最优实验条件下,该免疫传感器在检测范围:0.01~1.0 ng/mL和5~200 ng/mL内呈现良好线性关系,检测下限为0.003 ng/mL。结果表明,该免疫传感器灵敏度高,制备方法简易。
首先製備二氧化硅(SiO2)-殼聚糖(chitosan,Cs)納米複閤物。將其脩飾在玻碳電極錶麵,利用納米SiO2高的錶麵能和多孔結構吸附電子媒介體硫堇(Thi)。通過Thi中的氨基吸附納米金(nano-Au),從而固載甲胎蛋白抗體(anti-AFP),併用牛血清白蛋白(BSA)封閉非特異性吸附位點,成功製得一種新型的甲胎蛋白免疫傳感器。在最優實驗條件下,該免疫傳感器在檢測範圍:0.01~1.0 ng/mL和5~200 ng/mL內呈現良好線性關繫,檢測下限為0.003 ng/mL。結果錶明,該免疫傳感器靈敏度高,製備方法簡易。
수선제비이양화규(SiO2)-각취당(chitosan,Cs)납미복합물。장기수식재파탄전겁표면,이용납미SiO2고적표면능화다공결구흡부전자매개체류근(Thi)。통과Thi중적안기흡부납미금(nano-Au),종이고재갑태단백항체(anti-AFP),병용우혈청백단백(BSA)봉폐비특이성흡부위점,성공제득일충신형적갑태단백면역전감기。재최우실험조건하,해면역전감기재검측범위:0.01~1.0 ng/mL화5~200 ng/mL내정현량호선성관계,검측하한위0.003 ng/mL。결과표명,해면역전감기령민도고,제비방법간역。
First, the preparation of silica-chitosan nano composites. Then modify the surface of glassy carbon electrode, using nano silicon dioxide high porous structure and surface energy of adsorption electronic media body thionine. Through thionine amino adsorption of nanometer gold, which immobilized AFP antibody , using bovine serum albumin closed nonspecific adsorption sites, successfully obtained a new kind of AFP immune sensor. Under the optimal experimental conditions, the immune sensor detection range in 0.01~1.0 ng/mL and 5~200 ng/mL show good linear relations, detecting limit was 0.003 ng/mL. Results show that the sensor sensitivity, simple preparation method.