化学传感器
化學傳感器
화학전감기
Chemical Sensors
2015年
3期
44-49
,共6页
免疫传感器%癌胚抗原%纳米金%碳纳米管%明胶
免疫傳感器%癌胚抗原%納米金%碳納米管%明膠
면역전감기%암배항원%납미금%탄납미관%명효
immunosensor%CEA%nano-Au%MWCNTs%gelatin
利用纳米金与明胶-碳纳米管(MWCNTs)复合物固载癌胚抗体制得较高灵敏度的电流型癌胚抗原(CEA)免疫传感器。首先将制得的明胶-碳纳米管复合物修饰在金电极表面成膜，再利用明胶中丰富的氨基吸附纳米金，并通过静电吸附作用吸附癌胚抗体(anti-CEA)。实验过程中采用循环伏安法对电极的修饰过程及响应性能进行表征。结果表明，制得的免疫传感器对癌胚抗原有良好的电流响应，其线性范围为0．1~80．0 ng/mL，检出下限为0．03 ng/mL。
利用納米金與明膠-碳納米管(MWCNTs)複閤物固載癌胚抗體製得較高靈敏度的電流型癌胚抗原(CEA)免疫傳感器。首先將製得的明膠-碳納米管複閤物脩飾在金電極錶麵成膜，再利用明膠中豐富的氨基吸附納米金，併通過靜電吸附作用吸附癌胚抗體(anti-CEA)。實驗過程中採用循環伏安法對電極的脩飾過程及響應性能進行錶徵。結果錶明，製得的免疫傳感器對癌胚抗原有良好的電流響應，其線性範圍為0．1~80．0 ng/mL，檢齣下限為0．03 ng/mL。
이용납미금여명효-탄납미관(MWCNTs)복합물고재암배항체제득교고령민도적전류형암배항원(CEA)면역전감기。수선장제득적명효-탄납미관복합물수식재금전겁표면성막，재이용명효중봉부적안기흡부납미금，병통과정전흡부작용흡부암배항체(anti-CEA)。실험과정중채용순배복안법대전겁적수식과정급향응성능진행표정。결과표명，제득적면역전감기대암배항원유량호적전류향응，기선성범위위0．1~80．0 ng/mL，검출하한위0．03 ng/mL。
A highly sensitive amperometric immunosensor for the determination of carcinoembryonic antigen (CEA) was construted with the comprised of gelatin, muti-wall carbon nanotubes (MWCNTs). At first , place the prepared gelatin-carbon nanotube composites on the gold electrode surface. Then, absorb the nano-Au by the numerous amino of gelatin and anti-CEA by electrostatic adsorption on its surface successively . In the experiment,the perparing process of the immunosensor was characterized by the cyclic voltammetrys. The results show that the immunosensor present highly sensitivity, with a linear range of 0.1~80.0 ng/mL and a detection limit of 0.03 ng/mL.
