中国临床药理学杂志
中國臨床藥理學雜誌
중국림상약이학잡지
The Chinese Journal of Clinical Pharmacology
2015年
20期
2034-2036
,共3页
骨肉瘤%索拉菲尼%细胞凋亡%Lin28同系物A%微小核苷酸let-7d
骨肉瘤%索拉菲尼%細胞凋亡%Lin28同繫物A%微小覈苷痠let-7d
골육류%색랍비니%세포조망%Lin28동계물A%미소핵감산let-7d
osteosarcoma%sorafenib%apoptosis%lin -28 homolog A%microRNA let-7d
目的:研究索拉菲尼对骨肉瘤细胞增殖和凋亡的作用及其分子机制。方法将人骨肉瘤细胞(HOS细胞)分为对照组及索拉菲尼组,用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐比色法观察索拉菲尼对HOS细胞活力的影响,用蛋白质印迹法检测凋亡相关蛋白变化,用Real-time PCR检测let-7d变化情况。结果索拉菲尼可抑制HOS细胞活力,并呈剂量和浓度依赖性;同时,索拉菲尼可以抑制Lin28表达,同时促进let-7d表达;过表达Lin28可以逆转索拉菲尼的促凋亡作用。结论索拉菲尼通过调控Lin28/le-7d环路来促进骨肉瘤细胞凋亡。
目的:研究索拉菲尼對骨肉瘤細胞增殖和凋亡的作用及其分子機製。方法將人骨肉瘤細胞(HOS細胞)分為對照組及索拉菲尼組,用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴鹽比色法觀察索拉菲尼對HOS細胞活力的影響,用蛋白質印跡法檢測凋亡相關蛋白變化,用Real-time PCR檢測let-7d變化情況。結果索拉菲尼可抑製HOS細胞活力,併呈劑量和濃度依賴性;同時,索拉菲尼可以抑製Lin28錶達,同時促進let-7d錶達;過錶達Lin28可以逆轉索拉菲尼的促凋亡作用。結論索拉菲尼通過調控Lin28/le-7d環路來促進骨肉瘤細胞凋亡。
목적:연구색랍비니대골육류세포증식화조망적작용급기분자궤제。방법장인골육류세포(HOS세포)분위대조조급색랍비니조,용3-(4,5-이갑기새서-2)-2,5-이분기사담서추염비색법관찰색랍비니대HOS세포활력적영향,용단백질인적법검측조망상관단백변화,용Real-time PCR검측let-7d변화정황。결과색랍비니가억제HOS세포활력,병정제량화농도의뢰성;동시,색랍비니가이억제Lin28표체,동시촉진let-7d표체;과표체Lin28가이역전색랍비니적촉조망작용。결론색랍비니통과조공Lin28/le-7d배로래촉진골육류세포조망。
Objective To investigate the pro -apoptotic effect and mechanism of sorafenib on osteosarcoma.Methods HOS cells was divi-ded into control group and sorafenib group.3 -( 4 ,5 -dimethyl-2 -thiazolyl)-2,5-diphenyl-2 -H-tetrazolium bromide ( MTT) assay was chosen to investigate the effect of sorafenib on cell viability.Western blot was applied to detect the expression of proteins, and real -time RT-PCR were used to detect the expression of mRNAs.Results The results of MTT assay showed that sorafenib inhibit the proliferation of HOS cells in an dose-and time-dependent manner.Sorafenib induced the inhibition of lin-28 and increase of let-7d.Further study showed that Lin28 could attenuate the pro-apoptotic effect of sorafenibin HOS cells.Conclusion Sorafenib induces apoptosis of HOS cells by disrup-ting Lin28/let-7d feedback.