CAJ | 학술논문

目的：体外评价N－4－（二氯乙基）丁胺－1，8－萘酰亚胺（ XHH）对HepG2细胞的抑制作用及其作用机制。方法采用MTT法检测细胞增殖，高内涵筛选分析仪结合Annexin V－FITC／Hoechst33342， PI／Hoechst33342和Rh123／Hoechst33342双染色法检测细胞形态及膜电位；免疫荧光法检测caspase－3， caspase－9， Bcl－2， Bax的表达水平。结果 XHH能抑制HepG2细胞增殖，诱导细胞凋亡，降低线粒体膜电位，提高Bax／Bcl－2，使caspase－3， caspase－9表达增加。结论 XHH具有较好的抗HepG2肿瘤细胞作用，可通过线粒体途径诱导细胞凋亡。
목적：체외평개N－4－（이록을기）정알－1，8－내선아알（ XHH）대HepG2세포적억제작용급기작용궤제。방법채용MTT법검측세포증식，고내함사선분석의결합Annexin V－FITC／Hoechst33342， PI／Hoechst33342화Rh123／Hoechst33342쌍염색법검측세포형태급막전위；면역형광법검측caspase－3， caspase－9， Bcl－2， Bax적표체수평。결과 XHH능억제HepG2세포증식，유도세포조망，강저선립체막전위，제고Bax／Bcl－2，사caspase－3， caspase－9표체증가。결론 XHH구유교호적항HepG2종류세포작용，가통과선립체도경유도세포조망。
Objective To investigate the effects of 2-(4-di(2-chlo-roethyl) aminobutylamino)-1H-benz-[de]isoquinoline-1,3(2H)-dione( XHH) , a novel nitrogen mustard derivative, on the growth and apoptosis in human hepatoma HepG2 cells in vitro.Methods Cell pro-liferative effect was assessed by MTT assay.Changes of morphology and mitochondrial membrane potential ( MMP) were assessed by AnnexinV-FITC/Hoechst33342 , PI/Hoecsht33342 and Rh123/Hoechst33342 double staining using high content screening ( HCS ) .The expression of caspase-3 , caspase-9 , Bcl-2 , Bax was assessed by immunofluores-cence method using HCS. Results The results indicated that XHH could inhibit the proliferation of HepG2 cells, induce apoptosis, lose MMP, up-regulate the ratio of Bax/Bcl-2, increase the expression of caspase-3 , caspase-9.Conclusion XHH could inhibit proliferative of human hepatoma HepG2 cells and induce cell apoptosis via mitochon-drial pathway.