价值工程
價值工程
개치공정
Value Engineering
2015年
33期
208-209
,共2页
小麦酯酶%检出%有机磷农药
小麥酯酶%檢齣%有機燐農藥
소맥지매%검출%유궤린농약
wheat esterase%detection%organophosphorus pesticide
研究了小麦酯酶检测有机磷农药的残留分析方法,根据酶抑制法的原理,对反应温度、pH、反应时间、显色剂及样品提取液等条件进行优化,得出采用固兰B盐既兼顾了底物和显色剂双重作用,又具有底物稳定性好、灵敏、简便实用的特点,同时也体现出固兰B盐法的优越性,其最低检出浓度为0.1mg/kg.
研究瞭小麥酯酶檢測有機燐農藥的殘留分析方法,根據酶抑製法的原理,對反應溫度、pH、反應時間、顯色劑及樣品提取液等條件進行優化,得齣採用固蘭B鹽既兼顧瞭底物和顯色劑雙重作用,又具有底物穩定性好、靈敏、簡便實用的特點,同時也體現齣固蘭B鹽法的優越性,其最低檢齣濃度為0.1mg/kg.
연구료소맥지매검측유궤린농약적잔류분석방법,근거매억제법적원리,대반응온도、pH、반응시간、현색제급양품제취액등조건진행우화,득출채용고란B염기겸고료저물화현색제쌍중작용,우구유저물은정성호、령민、간편실용적특점,동시야체현출고란B염법적우월성,기최저검출농도위0.1mg/kg.
This article studies the analysis method of using wheat esterase to detect organophosphorus pesticide residues. According to the principle of enzymatic inhibition method, the reaction temperature, pH, reaction time, and the agent sample extracts are optimized. It is concluded that using fast blue salt B can not only considers the dual function of the substrate and the chromogenic agent, but also has the features of stable, sensitive and practical substrate, and at the same time the advantages of fast blue salt B are shown. Its minimum detectable concentration is 0.1mg/kg.
