CAJ | 학술논문

目的：采用葫芦素（JSI－124）阻断信号转导及转录激活因子3（STAT3）信号通路传导后，观察其对人食管癌Eca－109细胞增殖的影响。方法不同浓度的JSI－124（0、0.6、1.2、2.5、5.0μmol／L）处理Eca－109细胞后，利用CCK－8法检测细胞增殖水平、逆转录－聚合酶链反应法检测STAT3及下游细胞周期蛋白D1转录水平的表达、Western blot法检测食管癌Eca－109细胞中STAT3和抗凋亡因子bcl－2的表达以及使用流式细胞仪技术检测细胞的凋亡率的变化。结果在Eca－109细胞中，经JSI－124处理的Eca－109细胞中，STAT3的活化水平明显降低；同时随着JSI－124抑制浓度（0、1.2、2.5、5.0μmol／L）的增加，Eca－109细胞的总凋亡率也随之增加（3.52％、19.26％、39.89％、55.22％）（P ＜0.05）。结论JSI－124作为STAT3的选择性抑制剂，可显著抑制食管癌Eca－109细胞的增殖并促进细胞的凋亡。
목적：채용호호소（JSI－124）조단신호전도급전록격활인자3（STAT3）신호통로전도후，관찰기대인식관암Eca－109세포증식적영향。방법불동농도적JSI－124（0、0.6、1.2、2.5、5.0μmol／L）처리Eca－109세포후，이용CCK－8법검측세포증식수평、역전록－취합매련반응법검측STAT3급하유세포주기단백D1전록수평적표체、Western blot법검측식관암Eca－109세포중STAT3화항조망인자bcl－2적표체이급사용류식세포의기술검측세포적조망솔적변화。결과재Eca－109세포중，경JSI－124처리적Eca－109세포중，STAT3적활화수평명현강저；동시수착JSI－124억제농도（0、1.2、2.5、5.0μmol／L）적증가，Eca－109세포적총조망솔야수지증가（3.52％、19.26％、39.89％、55.22％）（P ＜0.05）。결론JSI－124작위STAT3적선택성억제제，가현저억제식관암Eca－109세포적증식병촉진세포적조망。
Objective To explore the effect of inhibition of signal transduction and activator of tran-scription 3 ( STAT3 ) with JSI-124 on the proliferation of Eca-109 cells of esophageal cancer.Methods After treatment with JSI-1244(0,0.6,1.2,2.5,5.0 μmol/L),CCK-8 assay was used to detect the inhibitory rate of Eca-109 cells, STAT3 and p-STAT3 protein levels were tested by Western blot and the levels of STAT3 mRNA were tested by reverse transcription-polymerase chain reaction in Eca-109 cells.The flow cytometry was used to detect the apoptosis rate of Eca-109 cells.Results STAT3 activation level was obvi-ously decreased in the JSI-124 treated Eca-109 cells.Along with the increase of the concentration level of JSI-1244(0,1.2,2.5,5.0 μmol/L),the cell apoptosis rate increased as well(3.52%,19.26%,39.89%, 55.22%) (P<0.05).Conclusion JSI-124,as a STAT selective inhibitor,inhibits the cell proliferation and enhances the apoptosis rate of Eca-109 cells.