CAJ | 학술논문

包涵体是指外源重组蛋白在细胞内凝集的无活性的固体颗粒。经分离、洗涤除去一些膜蛋白或膜碎片后，用适宜试剂溶解，如高pH值加低浓度的尿素缓冲液、不同羟基的醇类或含小分子的变性剂。传统的稀释、透析等复性方法效率一般较低，目前可通过高静水压、沸石的高通量筛选及高通量筛选结合实验设计软件的方法提高复性效率。希望可以找到一种适于所有重组蛋白的高效、简便的复性方法，实现不易获得的外源重组蛋白简易的规模化生产。
포함체시지외원중조단백재세포내응집적무활성적고체과립。경분리、세조제거일사막단백혹막쇄편후，용괄의시제용해，여고pH치가저농도적뇨소완충액、불동간기적순류혹함소분자적변성제。전통적희석、투석등복성방법효솔일반교저，목전가통과고정수압、비석적고통량사선급고통량사선결합실험설계연건적방법제고복성효솔。희망가이조도일충괄우소유중조단백적고효、간편적복성방법，실현불역획득적외원중조단백간역적규모화생산。
Inclusion bodies are the inactive solid particles agglutinating by exogenous recombinant pro-tein in cells.After separation and washing to remove some pieces of membrane proteins or film,they can be dissolved by appropriate reagent,for example the buffer with high pH value and low concentration of urea, alcohol with different hydroxyl or denaturant containing small molecule .The renaturation efficiency traditional renaturation method such as dilution,dialysis is generally low.At present,we can improve refolding efficiency through high pressure, high throughput screening of zeolite and high-throughput screening combined with experimental design software.We wish to find an efficient renaturation method suitable for all recombinant proteins,so as to realize large-scale production of recombinant proteins which are difficult to obtain .