蚌埠医学院学报
蚌埠醫學院學報
방부의학원학보
Journal of Bengbu Medical College
2015年
11期
1545-1547,1566
,共4页
王秀%王冬云%张文静%张劲%余婷婷%李见春
王秀%王鼕雲%張文靜%張勁%餘婷婷%李見春
왕수%왕동운%장문정%장경%여정정%리견춘
安妥沙星%高效液相色谱法%药代动力学
安妥沙星%高效液相色譜法%藥代動力學
안타사성%고효액상색보법%약대동역학
antofloxacin%high performance liquid chromatography%pharmacokinetics
目的::建立测定家兔血浆中安妥沙星浓度的高效液相色谱方法,探讨安妥沙星在家兔体内的药代动力学特征。方法:取安妥沙星片,按25 mg/kg剂量,研细,分散于0.5%羧甲基纤维素钠溶液中,给家兔灌胃,采血,血样经高氯酸处理后进行高效液相色谱法分析。色谱柱条件:Phenomenex C18柱(250 mm ×4.6 mm,4μm);乙腈:50 mmol/L枸橼酸溶液:1 mol/L醋酸胺溶液(19:80:1,体积比)为流动相,流速1.0 ml/min;检测波长为295 nm,柱温40℃。结果:安妥沙星在家兔血浆中的线性范围为0.164~10.5μg/ml,最低定量限为0.164μg/ml。日内及日间变异均<10%,准确度相对误差<5%。血浆中回收率>80%。实验条件下安妥沙星在家兔体内的药代动力学参数:血药浓度时间曲线下面积为(46.17±13.99)mg·L-1·h-1,达峰时间为(0.9±0.14)h,峰浓度为(5.15±0.54)mg/L,生物半衰期为(11.98±4.02)h,清除率为(0.58±0.19)L·kg-1·h-1。结论:该方法经考察符合生物样品的测定要求,可用于测定家兔血浆安妥沙星浓度和药代动力学的研究。
目的::建立測定傢兔血漿中安妥沙星濃度的高效液相色譜方法,探討安妥沙星在傢兔體內的藥代動力學特徵。方法:取安妥沙星片,按25 mg/kg劑量,研細,分散于0.5%羧甲基纖維素鈉溶液中,給傢兔灌胃,採血,血樣經高氯痠處理後進行高效液相色譜法分析。色譜柱條件:Phenomenex C18柱(250 mm ×4.6 mm,4μm);乙腈:50 mmol/L枸櫞痠溶液:1 mol/L醋痠胺溶液(19:80:1,體積比)為流動相,流速1.0 ml/min;檢測波長為295 nm,柱溫40℃。結果:安妥沙星在傢兔血漿中的線性範圍為0.164~10.5μg/ml,最低定量限為0.164μg/ml。日內及日間變異均<10%,準確度相對誤差<5%。血漿中迴收率>80%。實驗條件下安妥沙星在傢兔體內的藥代動力學參數:血藥濃度時間麯線下麵積為(46.17±13.99)mg·L-1·h-1,達峰時間為(0.9±0.14)h,峰濃度為(5.15±0.54)mg/L,生物半衰期為(11.98±4.02)h,清除率為(0.58±0.19)L·kg-1·h-1。結論:該方法經攷察符閤生物樣品的測定要求,可用于測定傢兔血漿安妥沙星濃度和藥代動力學的研究。
목적::건립측정가토혈장중안타사성농도적고효액상색보방법,탐토안타사성재가토체내적약대동역학특정。방법:취안타사성편,안25 mg/kg제량,연세,분산우0.5%최갑기섬유소납용액중,급가토관위,채혈,혈양경고록산처리후진행고효액상색보법분석。색보주조건:Phenomenex C18주(250 mm ×4.6 mm,4μm);을정:50 mmol/L구연산용액:1 mol/L작산알용액(19:80:1,체적비)위류동상,류속1.0 ml/min;검측파장위295 nm,주온40℃。결과:안타사성재가토혈장중적선성범위위0.164~10.5μg/ml,최저정량한위0.164μg/ml。일내급일간변이균<10%,준학도상대오차<5%。혈장중회수솔>80%。실험조건하안타사성재가토체내적약대동역학삼수:혈약농도시간곡선하면적위(46.17±13.99)mg·L-1·h-1,체봉시간위(0.9±0.14)h,봉농도위(5.15±0.54)mg/L,생물반쇠기위(11.98±4.02)h,청제솔위(0.58±0.19)L·kg-1·h-1。결론:해방법경고찰부합생물양품적측정요구,가용우측정가토혈장안타사성농도화약대동역학적연구。
Objective:To develop a high performance liquid chromatography method to assess the concentration of antofloxacin in rabbit plasma,and explore the drug metabolism of antofloxacin in rabbits Kinetic characteristics. Methods:Antofloxacin tablets were taken,powdered,added to 0. 5% sodium carboxymethyl cellulose solution and mixed. The solution was intragastric administrated to rabbits at a dose of 25 mg/kg. Blood samples were collected. Following protein precipitation, the analytes were separated on a Phenomenex C18 column(250 mm × 4. 6 mm,4 μm),with a mobile phase consisting of acetonitrile 50 mmol/L,citric acid solution 1 mmol/L,ammonium acetate solution(19:80:1,volume/volume ) at a flow rate of 1. 0 ml/ min,and the eluent was detected at 295 nm, column temperature was 40 ℃. Results:Linear calibration was generated over antofloxacin's concentration range of 0. 164 to 10. 5 μg/ml for plasma samples. Intra-and inter-days precision was <10%, and the accuracy of the relative error was <5%, which were acceptable for all quality control. Samples with relative errors were within the prescribed scopes, including the lowest limit of quantification of 0. 164 μg/ml. The mean recovery of antofloxacin from plasma was >80%. Pharmacokinetic parameters of antofloxacin in rabbits were estimated as follows:the area under concentration-time curve was(46. 17 ± 13. 99) mg·L-1 ·h-1 ,the time peak was (0. 9 ± 0. 14) h,the peak concentration was(5. 15 ± 0. 54) mg/L,the half time was(11. 98 ± 4. 02) h,and the clearance rate was (0. 58 ± 0. 19) L·kg-1 ·h-1 . Conclusions:This specific,sensitive and precise method is suitable for the pharmacokinetic study of antofloxacin in rabbits.