中国临床药理学杂志
中國臨床藥理學雜誌
중국림상약이학잡지
The Chinese Journal of Clinical Pharmacology
2015年
21期
2164-2169
,共6页
李德强%肖媛媛%戴荣源%张志清%单爱莲
李德彊%肖媛媛%戴榮源%張誌清%單愛蓮
리덕강%초원원%대영원%장지청%단애련
活性成分%筛选%中药%靶标%靶分子亲和-质谱
活性成分%篩選%中藥%靶標%靶分子親和-質譜
활성성분%사선%중약%파표%파분자친화-질보
bioactive compound%screening%traditional Chinese medi-cines%target%target molecule affinity coupled with mass spectrometry
中药效应成分的快速辨识是现代药物研究的重要挑战之一，本文对2005年以来适用于中药等复杂体系中活性成分筛选的靶分子亲和－质谱联用技术进行了综述。根据亲和筛选的原理，将对柱前亲和筛选（包括超滤－质谱联用技术、分子排阻色谱－质谱联用技术、涡流色谱－质谱联用技术、限制介入－质谱联用技术和固定化靶分子－质谱联用技术）和柱后亲和筛选（液相色谱柱后在线反应－质谱联用技术）的方法及其应用进行综述，为中药等复杂体系中活性成分的快速筛选提供技术参考。
中藥效應成分的快速辨識是現代藥物研究的重要挑戰之一，本文對2005年以來適用于中藥等複雜體繫中活性成分篩選的靶分子親和－質譜聯用技術進行瞭綜述。根據親和篩選的原理，將對柱前親和篩選（包括超濾－質譜聯用技術、分子排阻色譜－質譜聯用技術、渦流色譜－質譜聯用技術、限製介入－質譜聯用技術和固定化靶分子－質譜聯用技術）和柱後親和篩選（液相色譜柱後在線反應－質譜聯用技術）的方法及其應用進行綜述，為中藥等複雜體繫中活性成分的快速篩選提供技術參攷。
중약효응성분적쾌속변식시현대약물연구적중요도전지일，본문대2005년이래괄용우중약등복잡체계중활성성분사선적파분자친화－질보련용기술진행료종술。근거친화사선적원리，장대주전친화사선（포괄초려－질보련용기술、분자배조색보－질보련용기술、와류색보－질보련용기술、한제개입－질보련용기술화고정화파분자－질보련용기술）화주후친화사선（액상색보주후재선반응－질보련용기술）적방법급기응용진행종술，위중약등복잡체계중활성성분적쾌속사선제공기술삼고。
Rapid screening and characterization of bioactive compounds from traditional Chinese medicines ( TCM) is one of the highlighted re-search challenges in the field of drug development.Target molecule affin-ity coupled with mass spectrometry ( MS) has become a new method for the screening of bioactive compounds from complex mixture, the recent development of which since 2005 will be reviewed.According to the prin-ciple of affinity screening, the methods could be classified into pre -column affinity screening including ultrafiltration -MS, size -exclusion chromatography-MS, turbulent-flow chromatography-MS, restricted-access material-MS and immobilized target-MS and post-column af-finity screening (HPLC-continuous-flow bioassay-MS).Each meth-od with its typical applications would be introduced, providing technique references for the screening of bioactive compounds from complex mixture such as TCM.
