CAJ | 학술논문

目的：探讨脊髓挫伤、脊髓横断及持续性占位损伤对大鼠继发性神经细胞凋亡的影响作用。方法：选取健康雄性Wistar大鼠66只，进行编号后采用随机数字表法分为A（18只，脊髓挫伤）、B（18只，脊髓横断）、C（18只，持续性占位）、D（6只、假手术组）、E（6只、对照组）五组，分别观察伤后1、4、7d 各组大鼠的神经细胞凋亡指数、脊髓组织中Bcl-2、Bax、caspase-3蛋白的表达情况。结果：A、B、C三组大鼠在造模后第1天均出现脊髓灰质和白质染色阳性标记，且三组大鼠的灰质、白质神经细胞凋亡指数差异具有统计学意义（ P＜0.05）；在造模后第4、7天三组大鼠的灰质、白质区域脊髓凋亡细胞指数均呈现出增高趋势（P＜0.05）；在造模后第1、4、7天C组大鼠的脊髓灰质、白质区域凋亡细胞指数显著高于A组、B组，B组显著高于A组且差异均具有统计学意义（P＜0.05）。造模后第1、4、7天A、B、C、D、E组五组大鼠的Bcl-2、Bax、caspase-3蛋白表达差异均具有统计学意义（P＜0.05），造模后第1、4、7天A、B、C三组大鼠的Bcl-2、Bax、caspase-3蛋白表达显著高于D组和E组（ P＜0.05）。结论：大鼠脊髓损伤后神经细胞继发性凋亡显著，严重程度与损伤类型有关。
목적：탐토척수좌상、척수횡단급지속성점위손상대대서계발성신경세포조망적영향작용。방법：선취건강웅성Wistar대서66지，진행편호후채용수궤수자표법분위A（18지，척수좌상）、B（18지，척수횡단）、C（18지，지속성점위）、D（6지、가수술조）、E（6지、대조조）오조，분별관찰상후1、4、7d 각조대서적신경세포조망지수、척수조직중Bcl-2、Bax、caspase-3단백적표체정황。결과：A、B、C삼조대서재조모후제1천균출현척수회질화백질염색양성표기，차삼조대서적회질、백질신경세포조망지수차이구유통계학의의（ P＜0.05）；재조모후제4、7천삼조대서적회질、백질구역척수조망세포지수균정현출증고추세（P＜0.05）；재조모후제1、4、7천C조대서적척수회질、백질구역조망세포지수현저고우A조、B조，B조현저고우A조차차이균구유통계학의의（P＜0.05）。조모후제1、4、7천A、B、C、D、E조오조대서적Bcl-2、Bax、caspase-3단백표체차이균구유통계학의의（P＜0.05），조모후제1、4、7천A、B、C삼조대서적Bcl-2、Bax、caspase-3단백표체현저고우D조화E조（ P＜0.05）。결론：대서척수손상후신경세포계발성조망현저，엄중정도여손상류형유관。
Objective:To study the spinal cord injury, spinal cord transection and persistent placeholder damage on the influence of secondary neural cell apoptosis in rats.Methods: Select 60 healthy male Wistar rats, numbered after using the random number table method is divided into A (18,spinal cord contusion),B (18,spinal cord transection),C (18,continuous placeholder),D (6,control),E (6,the control group only) groups of five,were observed at the 1,4,7 D after 5 group of rats nerve cell apoptosis index, spinal cord tissue Bcl-2,the expression of Bax,caspase 3 protein.Results:A,B,C three groups of rats after building 1 d are gray and white matter positive markers, and the gray matter and white matter of three groups of rats nerve cell apoptosis index differences statistically significant ( P<0.05);4 d,7 d after building gray matter and white matter of three groups of rats tend to place increased ap-optotic cells in the spinal cord index ( P<0.05);in building 1,4,7 d group C after rat spinal cord grey matter and white matter of apoptotic cell index was significantly higher than that of group A and group B, group B were significantly higher in group A and the differences were statistically significant (P<0.05).1,4,7 d after building A,B,C,D,E five group rats the Bcl-2,Bax,caspase-3 protein expression differences were statistically significant (P<0.05),1,4,7 d after building A,B,C the Bcl-2 of three groups of rats, Bax,caspase-3 protein expression was significantly higher than that of group D and group E ( P<0.05).Conclusion: Secondary rats after spinal cord injury of nerve cells apoptosis,apoptosis time,severity,and damage type and severity.