CAJ | 학술논문

将常规免疫组织化学法与表面增强拉曼散射光谱( SERS)结合,建立了一种简便、快速测定大鼠脑组织中神经元特异性骨架蛋白的新方法。将所制备的SERS免疫胶体金标签加入至大鼠脑组织冰冻切片,研究了经免疫识别后组织的SERS光谱,在脑组织切片中得到了探针分子对巯基苯甲酸(MBA)的特征峰(1076,1589 cm-1)。对比了不同浓度抗体对SERS信号及免疫组化染色的影响,结果表明,与传统的免疫组化法相比, SERS法可以简化步骤,缩短实验时间,同时展现出更高的检测灵敏度和选择性。
장상규면역조직화학법여표면증강랍만산사광보( SERS)결합,건립료일충간편、쾌속측정대서뇌조직중신경원특이성골가단백적신방법。장소제비적SERS면역효체금표첨가입지대서뇌조직빙동절편,연구료경면역식별후조직적SERS광보,재뇌조직절편중득도료탐침분자대구기분갑산(MBA)적특정봉(1076,1589 cm-1)。대비료불동농도항체대SERS신호급면역조화염색적영향,결과표명,여전통적면역조화법상비, SERS법가이간화보취,축단실험시간,동시전현출경고적검측령민도화선택성。
Surface-enhanced Raman scattering( SERS) was used to develop and optimize a novel, quick and simple immunohistochemical ABC ( avidin-biotin-HRP complex ) assay for detecting specific skeleton protein neurofilament-200(NF-200) in the frozen sections of rat brain tissue. In this study, anti mouse NF-200 la-beled colloid gold was used as immunoprobe, which was added into the frozen sections of rat brain tissue con-taining NF-200 protein. Intense SERS signals of p-mercaptobenzoic acid( MBA) at 1076 and 1589 cm-1 were observed clearly after the specific binding of antigen and antibody. Meanwhile, the SERS spectra and the im-munohistochemical staining effect of antigen and antibody complex with different concentrations of anti NF-200 were examined. Compared with the traditional method, this assay can simplify the procedure, moreover, it showed higher sensitivity and selectivity.