CAJ | 학술논문

目的：探讨下调 EZH2基因表达对人结肠癌细胞凋亡、增殖的影响。方法以人结肠癌细胞HCT116为研究材料，设计合成针对EZH2基因mRNA的小干扰 RNA （ si －EZH2）， lipofectamine 2000脂质体转染 si －EZH2下调HCT116细胞株EZH2蛋白表达。蛋白免疫印迹法检测下调后EZH2蛋白表达水平，流式细胞术检测下调 EZH2后细胞凋亡水平，噻唑蓝（ MTT ）法检测HCT116细胞增殖能力变化。结果 si －EZH2可显著下调 EZH2蛋白表达（ P＜0．05）；下调EZH2蛋白表达后细胞凋亡比例增加，结肠癌细胞的增殖能力明显降低（ P＜0．05）。结论 EZH2可能参与了人结肠癌细胞增殖与凋亡过程，有望成为结肠癌药物治疗新靶点。
목적：탐토하조 EZH2기인표체대인결장암세포조망、증식적영향。방법이인결장암세포HCT116위연구재료，설계합성침대EZH2기인mRNA적소간우 RNA （ si －EZH2）， lipofectamine 2000지질체전염 si －EZH2하조HCT116세포주EZH2단백표체。단백면역인적법검측하조후EZH2단백표체수평，류식세포술검측하조 EZH2후세포조망수평，새서람（ MTT ）법검측HCT116세포증식능력변화。결과 si －EZH2가현저하조 EZH2단백표체（ P＜0．05）；하조EZH2단백표체후세포조망비례증가，결장암세포적증식능력명현강저（ P＜0．05）。결론 EZH2가능삼여료인결장암세포증식여조망과정，유망성위결장암약물치료신파점。
Objective To explore the effect of down regulation of EZH2 expression by shRNA on apoptosis and proliferation in colorectal cancer cell.Methods The human colorectal cancer cell line HCT116 was used as the materials.The EZH2 expression was knockdown by traninfecting siRNA.The EZH2 protein expression was tested by western-blot array, the HCT116 apoptosis rate was tested by flow cytometry array and the HCT116 cell proliferation ability was tested by MTT array.Results The EZH2 protein significant decreased by si -EZH2 ( P <0.05 ) .The apoptosis rate significant increased by knockdown the EZH2 expression ( P<0.05) .And the proliferation ability was inhibited after knockdown the EZH2 expression ( P<0.05).Conclusion EZH2 may be involved in the process of apoptosis and proliferation in human colorectal cancer cells which could be a potential target for treatment.