CAJ | 학술논문

利用60 Co-γ射线辐射诱变籼稻恢复系中恢8015,获得了一份窄卷叶突变体 Nrl3(t).与野生型中恢8015相比,突变体叶片显著变窄内卷、株高降低、穗长变短、结实率降低、千粒重降低、粒长粒宽减小.细胞学分析表明,突变体维管束减少和泡状细胞不够饱满是导致叶片变窄卷曲的原因.遗传分析表明该突变体受一对隐性核基因控制,以窄卷叶突变体Nrl3(t )与粳稻品种02428杂交获得的 F2分离群体作为定位群体,利用 SSR 标记和新开发的 InDel 标记,将窄卷叶基因Nrl3(t)定位于第2染色体长臂标记 C9和 C10E 之间约70．3 kb 区间内.研究结果为该基因的克隆及进一步揭示水稻窄卷叶形成的分子机理奠定了基础.
이용60 Co-γ사선복사유변선도회복계중회8015,획득료일빈착권협돌변체 Nrl3(t).여야생형중회8015상비,돌변체협편현저변착내권、주고강저、수장변단、결실솔강저、천립중강저、립장립관감소.세포학분석표명,돌변체유관속감소화포상세포불구포만시도치협편변착권곡적원인.유전분석표명해돌변체수일대은성핵기인공제,이착권협돌변체Nrl3(t )여갱도품충02428잡교획득적 F2분리군체작위정위군체,이용 SSR 표기화신개발적 InDel 표기,장착권협기인Nrl3(t)정위우제2염색체장비표기 C9화 C10E 지간약70．3 kb 구간내.연구결과위해기인적극륭급진일보게시수도착권협형성적분자궤리전정료기출.
Nrl3 (t )was a novel narrow and rolled leaf mutant from the indica restorer line Zhonghui 801 5 after exposure to 60 Co-γray.Nrl3 (t )exhibited significant adaxially-rolled leaves,reduced plant height,shortened panicle length, reduced grain length,grain width and thousand-grain weight,as well as the seed-setting rate.Cytological analysis showed that the rolled leaf phenotype was due to the reduced number of vascular bundleand the changed size of bulliform cells.In an initial mapping with F2 individuals derived from a cross between Nrl3 (t)and japonica 02428,the narrow and rolled leaf gene Nrl3 (t)was eventually mapped to a 70.3 kb region on the long arm of chromosome 2 between two newly developed sequence tagged site markers C9 and C10E.These results would lay a good foundation for molecular cloning and functional analysis of Nrl3 (t).