水稻窄叶突变体 nal10的鉴定与基因精细定位
수도착협돌변체 nal10적감정여기인정세정위
Identification and Fine Mapping of a Narrow Leaf Mutant nal10 in Rice
  • 万方数据
    中国水稻科学 중국수도과학
    Chinese Journal of Rice Science
    2015年 6期 587-594 ,共8页

    方云霞%朱丽%潘江杰%余海平%薛大伟%饶玉春%王小虎%石珍源%张栋%祝阳舟%董国军%曾大力%张光恒%郭龙彪%胡江%钱前

    방운하%주려%반강걸%여해평%설대위%요옥춘%왕소호%석진원%장동%축양주%동국군%증대력%장광항%곽룡표%호강%전전

    水稻%窄叶%NAL10%基因定位 水稻%窄葉%NAL10%基因定位
    수도%착협%NAL10%기인정위
    rice%narrow leaf%NAL10%gene mapping
    利用 EMS 诱变粳稻品种武运粳7号获得一个新窄叶突变体 nal10,该突变体在苗期表现出极窄叶和弱小的特征,在生殖期表现出矮化、窄叶、畸颖和不育的表型.组织解剖学分析表明,nal10的叶片窄化可能是由于叶片大脉和小脉数量减少造成的.遗传分析表明,该窄叶表型受一对隐性核基因控制.利用 nal10与台中本地一号(TN1)构建的 F2群体,通过混合分离法,在第1染色体上找到3个紧密连锁的 SSR 标记(RM1287、RM562和 RM5638).进一步利用新发展的分子标记,最终将该基因定位在 STS 标记 PK83和 PK78之间的55．2 kb 范围内,该区间共有8个开放阅读框.RT-PCR 分析表明, NAL10的突变能够造成生长素生物合成、信号转导和运输基因转录水平的下降,因此 NAL10是一个与生长素相关的窄叶新基因.这些结果为进一步克隆该基因、丰富水稻叶发育的遗传调控网络打下了基础.
    이용 EMS 유변갱도품충무운갱7호획득일개신착협돌변체 nal10,해돌변체재묘기표현출겁착협화약소적특정,재생식기표현출왜화、착협、기영화불육적표형.조직해부학분석표명,nal10적협편착화가능시유우협편대맥화소맥수량감소조성적.유전분석표명,해착협표형수일대은성핵기인공제.이용 nal10여태중본지일호(TN1)구건적 F2군체,통과혼합분리법,재제1염색체상조도3개긴밀련쇄적 SSR 표기(RM1287、RM562화 RM5638).진일보이용신발전적분자표기,최종장해기인정위재 STS 표기 PK83화 PK78지간적55．2 kb 범위내,해구간공유8개개방열독광.RT-PCR 분석표명, NAL10적돌변능구조성생장소생물합성、신호전도화운수기인전록수평적하강,인차 NAL10시일개여생장소상관적착협신기인.저사결과위진일보극륭해기인、봉부수도협발육적유전조공망락타하료기출.
    A novel rice mutant narrow leaf 10 (nal10 )was identified from an EMS (ethyl methane sulphonate) mutagenized population of Wuyunjing 7.The mutant was characterized by narrow leaves and smaller architecture phenotype at the seedling stage,and dwarf,narrow leaves,deformed flowers and male sterility at the reproduction stage.Cyto-anatomical analysis indicated that the mutant had fewer large and small veins compared with the wild-type, which may account for the narrow leaf phenotype.The genetic analysis indicated the narrow leaf trait was controlled by a recessive nuclear gene.Using F2 population derived from the cross of nal10 and Taizhongbendi 1 ,three closely linked SSR markers RM1287,RM5 62 and RM5 638 on chromosome 1 were obtained by the bulked segregant analysis method. With the developed new markers,the gene was finally fine-mapped to an interval of 55 .2 kb between the STS markers PK83 and PK78,where a total of eight ORFs were found.RT-PCR analysis indicated the expression levels of auxin biosynthesis,signal transduction and transport related genes were all down-regulated,suggesting NAL10 is a new narrow leaf gene involving in the auxin metabolism.These results laid a foundation for further cloning of the gene and enrich the genetic regulation network of leaf development in rice.
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