海峡药学
海峽藥學
해협약학
Strait Pharmaceutical Journal
2015年
10期
55-57
,共3页
蜂胶%总黄酮%分光光度法%含量
蜂膠%總黃酮%分光光度法%含量
봉효%총황동%분광광도법%함량
Propolis%Total flavonoids%Spectrophotometry%Content
目的 检测目前部分市售蜂胶胶囊中黄酮类化合物的含量,为蜂胶及其制剂的质量标准提供可靠的理论依据,同时也为消费者购买蜂胶产品提供参考. 方法 用乙醇作为提取溶剂,超声波法提取样品中黄酮成分. 以芦丁为对照品,NaNO2-Al(NO3)3-NaOH为显色剂,用紫外可见分光光度法在500nm处测定蜂胶产品中黄酮类化合物的含量.结果 9种蜂胶样品总黄酮含量高低顺序排列为07>06>03>05>04>02>01>08 ,09号样品未检测到黄酮. 结论 该方法 方便准确,重现性好,测定结果 能反应客观实际,可作为蜂胶胶囊的质量标准. 市售蜂胶黄酮含量参差不齐,品质差异较大.
目的 檢測目前部分市售蜂膠膠囊中黃酮類化閤物的含量,為蜂膠及其製劑的質量標準提供可靠的理論依據,同時也為消費者購買蜂膠產品提供參攷. 方法 用乙醇作為提取溶劑,超聲波法提取樣品中黃酮成分. 以蘆丁為對照品,NaNO2-Al(NO3)3-NaOH為顯色劑,用紫外可見分光光度法在500nm處測定蜂膠產品中黃酮類化閤物的含量.結果 9種蜂膠樣品總黃酮含量高低順序排列為07>06>03>05>04>02>01>08 ,09號樣品未檢測到黃酮. 結論 該方法 方便準確,重現性好,測定結果 能反應客觀實際,可作為蜂膠膠囊的質量標準. 市售蜂膠黃酮含量參差不齊,品質差異較大.
목적 검측목전부분시수봉효효낭중황동류화합물적함량,위봉효급기제제적질량표준제공가고적이론의거,동시야위소비자구매봉효산품제공삼고. 방법 용을순작위제취용제,초성파법제취양품중황동성분. 이호정위대조품,NaNO2-Al(NO3)3-NaOH위현색제,용자외가견분광광도법재500nm처측정봉효산품중황동류화합물적함량.결과 9충봉효양품총황동함량고저순서배렬위07>06>03>05>04>02>01>08 ,09호양품미검측도황동. 결론 해방법 방편준학,중현성호,측정결과 능반응객관실제,가작위봉효효낭적질량표준. 시수봉효황동함량삼차불제,품질차이교대.
OBJECTIVE By determining the content of flavonoids in propolis capsul to provide a reliable theo-retical basis for the quality standards,and also a reference for consumers to buy propolis.METHODS Use ethanol as a solvent extraction,ultrasound extraction for the extraction of flavonoids.Rutin was used for a reference substance and NaNO2-Al( NO3 ) 3-NaOH as color reagent.UV Spectrophotomet was employed to determine the content of fla-vonoids products in propolis products and the samples were determined at 500 nm.RESULTS The content of total flavonoids in order of frequency for 9 propolis samples was 07>06>03>05>04>02>01>08 ,09 samples were not detected flavonoids.CONCLUSION The method is easy-to-handle with good accuratcy and reproduciblity,and the result can objectively reflect the reality.It can be used as the quality standards of propolis Capsul.The content of flavonoids in propolis sample is uneven.They have big difference in quality.
