海峡药学
海峽藥學
해협약학
Strait Pharmaceutical Journal
2015年
10期
34-36
,共3页
垂盆草颗粒%异鼠李素%HPLC
垂盆草顆粒%異鼠李素%HPLC
수분초과립%이서리소%HPLC
Stringy Stonecrop Herb Granules%Isohamnetin%HPLC
目的 建立垂盆草颗粒中异鼠李素的HPLC的测定方法 ,为制定该制剂质量标准中定量测定方法 及限度提供依据. 方法 色谱柱:Sun-fire–C18(4.6mm ×150mm,5μm) 流动相:以甲醇-0.4%磷酸溶液(50:50);流速:1.0mL· min -1,检测波长:360nm,柱温:25℃,进样量:10μL.结果 异鼠李素的线性范围为12.39~154.94μg,平均加样回收率(n=6)分别为99.26%、99.33%和99.10%,RSD为0.77%. 结论 该方法 简单、快速、结果 准确,重现性好,为垂盆草颗粒中异鼠李素的测定提供可靠的方法 .
目的 建立垂盆草顆粒中異鼠李素的HPLC的測定方法 ,為製定該製劑質量標準中定量測定方法 及限度提供依據. 方法 色譜柱:Sun-fire–C18(4.6mm ×150mm,5μm) 流動相:以甲醇-0.4%燐痠溶液(50:50);流速:1.0mL· min -1,檢測波長:360nm,柱溫:25℃,進樣量:10μL.結果 異鼠李素的線性範圍為12.39~154.94μg,平均加樣迴收率(n=6)分彆為99.26%、99.33%和99.10%,RSD為0.77%. 結論 該方法 簡單、快速、結果 準確,重現性好,為垂盆草顆粒中異鼠李素的測定提供可靠的方法 .
목적 건립수분초과립중이서리소적HPLC적측정방법 ,위제정해제제질량표준중정량측정방법 급한도제공의거. 방법 색보주:Sun-fire–C18(4.6mm ×150mm,5μm) 류동상:이갑순-0.4%린산용액(50:50);류속:1.0mL· min -1,검측파장:360nm,주온:25℃,진양량:10μL.결과 이서리소적선성범위위12.39~154.94μg,평균가양회수솔(n=6)분별위99.26%、99.33%화99.10%,RSD위0.77%. 결론 해방법 간단、쾌속、결과 준학,중현성호,위수분초과립중이서리소적측정제공가고적방법 .
OBJECTIVE To establish a method for determing isohamnetin in Stringy Stonecrop Herb Granules by HPLC.METHODS The HPLC method was carried out on Agilent C18 column(4.6mm ×150mm,5μm),The mobile phase was methanol-0.5%phosphate(50:50);The flow rate was 1.0mL· min-1 ,The detection wavelength was at 360nm,The column temperature was set at 25℃,and 10μL injection volumn.RESULTS There was a good linear relationship when isohamnetin were in the range of 12.39 ~154.94μg.The average recoveriy was 99.10%· 34·.RSD were 0.77%.CONCLUSION The method is simple in operation,high in sensitivity and good in reproduc-ibility,and can be used for the determination the isohamnetin in Stringy Stonecrop Herb Granules by HPLC.
