CAJ | 학술논문

抗性验证试验表明籼稻大白谷抗水稻干尖线虫侵染。为进一步探究此抗性与病程相关蛋白———半胱氨酸蛋白酶抑制剂(Cystatin)的相关性,基于水稻基因组数据克隆籼稻大白谷 Cystatin 家族的 OC-XI I 基因,分别进行 OC-XII、J aponica-OC-XII 和 Indica-OC-XII 蛋白质三维结构构建,并与水稻干尖线虫组织蛋白酶 B(Cathepsin B)进行分子对接以验证 OC-XI I 基因编码蛋白对 Cathepsin B 的抑制功能。通过荧光定量 qPCR 验证 OC-XI I 基因在线虫侵染前后的表达特性,OC-XII蛋白可以与水稻干尖线虫 Cathepsin B 蛋白质特异结合,线虫侵染后 OC-XI I 基因在浸染12h~2d 期间表达量持续上调,侵染3d 后下调。表明 OC-XI I 基因在大白谷抗水稻干尖线虫侵染过程中发挥功能,具有对植物寄生线虫进行生物防治的潜力,有必要对其进行深入研究。
항성험증시험표명선도대백곡항수도간첨선충침염。위진일보탐구차항성여병정상관단백———반광안산단백매억제제(Cystatin)적상관성,기우수도기인조수거극륭선도대백곡 Cystatin 가족적 OC-XI I 기인,분별진행 OC-XII、J aponica-OC-XII 화 Indica-OC-XII 단백질삼유결구구건,병여수도간첨선충조직단백매 B(Cathepsin B)진행분자대접이험증 OC-XI I 기인편마단백대 Cathepsin B 적억제공능。통과형광정량 qPCR 험증 OC-XI I 기인재선충침염전후적표체특성,OC-XII단백가이여수도간첨선충 Cathepsin B 단백질특이결합,선충침염후 OC-XI I 기인재침염12h~2d 기간표체량지속상조,침염3d 후하조。표명 OC-XI I 기인재대백곡항수도간첨선충침염과정중발휘공능,구유대식물기생선충진행생물방치적잠력,유필요대기진행심입연구。
The previous resistance verification indicated that Dabaigu (Oryza sativa ssp.indica)resists the infection of Aphelenchoides bessey .To reveal the interaction between the resistance and the pathogenesis-related protein Cystatin, the gene OC-X I I was cloned,the docking between OC-XII and A .besseyi Cathepsin B was done,and the expression of OC-X I I was tested by quantitative real-time PCR.Results showed that OC-XII bound with Cathepsin B specifily.The expression level of OC-X I I was upregulated from 12 hours after infection to 2 days after infection,and downregulated 3 days after infection.These results indicated that OC-X I I was involved in the resistance to A .besseyi .The gene has the potential for biological control of plant-parasitic nematodes,and it is necessary to conduct in-depth research.