CAJ | 학술논문

在粳稻品种 Dongjin 大田种植过程中,发现一个黄绿叶自然突变体,命名为 dj yg .该突变体在苗期表现明显的黄绿叶表型,抽穗以后,叶色逐渐恢复正常.叶绿素含量测定结果表明,在苗期、分蘖盛期及抽穗期叶绿素 b 的含量分别下降53％、62％、36％.电镜结果表明,分蘖期突变体中基粒、类囊体垛堆凌乱、排列疏松,类囊体基质较为稀薄.qRT-PCR 结果证实,PORA 、Cab1R 、PsbA 的表达量在突变体中均较野生型明显下调.遗传分析结果表明,黄绿叶突变体 dj yg 由一对隐性主效核基因控制,图位克隆确定该候选基因为编码叶绿素合成酶基因YGL1的一个新等位基因.该突变体未影响植株的主要农艺性状,可作为一个理想的表型标记应用于杂交稻育种工作中.
재갱도품충 Dongjin 대전충식과정중,발현일개황록협자연돌변체,명명위 dj yg .해돌변체재묘기표현명현적황록협표형,추수이후,협색축점회복정상.협록소함량측정결과표명,재묘기、분얼성기급추수기협록소 b 적함량분별하강53％、62％、36％.전경결과표명,분얼기돌변체중기립、류낭체타퇴릉란、배렬소송,류낭체기질교위희박.qRT-PCR 결과증실,PORA 、Cab1R 、PsbA 적표체량재돌변체중균교야생형명현하조.유전분석결과표명,황록협돌변체 dj yg 유일대은성주효핵기인공제,도위극륭학정해후선기인위편마협록소합성매기인YGL1적일개신등위기인.해돌변체미영향식주적주요농예성상,가작위일개이상적표형표기응용우잡교도육충공작중.
A spontaneous yellow-green mutant was obtained from the japonica rice cultivar Dongjin in the field, designated as dj yg .The mutant showed obvious yellow-green leaf phenotype at the seedling stage but the chlorotic leaves returned to green after the heading stage.Consistent with the phenotype,the chlorophyll b content of the mutant was reduced by 53%,62% and 36% at the seedling,tillering and heading stages,respectively,compared with that of the wild type (WT).At the tillering stage,we observed that the granas and thylakoid stacks displayed a mess and loose structure,as well as less density in the mutant dj yg compared with the WT under a transmission electron microscopy. The transcriptional levels of PORA ,Cab1R ,PsbA were also decreased in the mutant dj yg .Genetic analysis and map-based cloning demonstrated that the phenotype of the mutant was controlled by a major recessive nuclear gene,which is an allele of chlorophyll synthase YGL1 .The mutant dj yg can be used as a phenotypic marker in rice breeding program since the main agronomic traits of the mutant dj yg are normal compared with those of the WT.