海峡药学
海峽藥學
해협약학
Strait Pharmaceutical Journal
2015年
10期
47-49
,共3页
美人草%酶解%渗漉%莨菪碱
美人草%酶解%滲漉%莨菪堿
미인초%매해%삼록%랑탕감
Beauty herbal%Enzymolysis%Percolation%Hyoscyamine
目的 应用酶渗漉法和醇渗漉法分别提取美人草(颠茄草),考察两种溶剂提取对其中主要活性物质莨菪碱的提取率,优选最佳提取方法 . 方法 用pH值为2.0、体外消化活力为24~30μ· mL -1的胃蛋白酶溶液为溶剂渗漉美人草,以80%乙醇渗漉液做对照,用高效液相色谱法测定两组提取液中莨菪碱的含量,比较其提取率. 结果 酶渗漉提取法莨菪碱提取率为4.79mg· g-1,醇渗漉提取法提取率为3.52mg· g -1 ,酶渗漉法比醇渗漉法提取率高36.1%. 结论 酶渗漉法提取美人草能显著提高主要活性物质莨菪碱的提取率,可用于美人草活性物质的提取.
目的 應用酶滲漉法和醇滲漉法分彆提取美人草(顛茄草),攷察兩種溶劑提取對其中主要活性物質莨菪堿的提取率,優選最佳提取方法 . 方法 用pH值為2.0、體外消化活力為24~30μ· mL -1的胃蛋白酶溶液為溶劑滲漉美人草,以80%乙醇滲漉液做對照,用高效液相色譜法測定兩組提取液中莨菪堿的含量,比較其提取率. 結果 酶滲漉提取法莨菪堿提取率為4.79mg· g-1,醇滲漉提取法提取率為3.52mg· g -1 ,酶滲漉法比醇滲漉法提取率高36.1%. 結論 酶滲漉法提取美人草能顯著提高主要活性物質莨菪堿的提取率,可用于美人草活性物質的提取.
목적 응용매삼록법화순삼록법분별제취미인초(전가초),고찰량충용제제취대기중주요활성물질랑탕감적제취솔,우선최가제취방법 . 방법 용pH치위2.0、체외소화활력위24~30μ· mL -1적위단백매용액위용제삼록미인초,이80%을순삼록액주대조,용고효액상색보법측정량조제취액중랑탕감적함량,비교기제취솔. 결과 매삼록제취법랑탕감제취솔위4.79mg· g-1,순삼록제취법제취솔위3.52mg· g -1 ,매삼록법비순삼록법제취솔고36.1%. 결론 매삼록법제취미인초능현저제고주요활성물질랑탕감적제취솔,가용우미인초활성물질적제취.
ABJECTIVE Enzyme percolation method and alcohol percolation method dwere utilized to extract beauty herbal( Atropa belladonna L).Extraction rates of the main active substance-hyoscyamine by two methods were investigated.The bestter extraction method was selected.METHODS In the experimental group, the pepsin solu-tion,pH was 2.0,in vitro digestion energy was 24~30μ· mL-1 ,was utilized as solvent to percolate beauty herbal.In the control group,the 80%ethanol solution was utilized to percolate beauty herbal.High performance liquid chroma-tography was utilized to test the content of hyoscyamine in extract of two groups and extraction rates of two groups were compared.RESULTS The extraction rate of hyoscyamine in experimental group was 4.79 mg · g-1 , it was 36.1%higher than that of control group which was 3.52 mg · g-1 .CONCLUSION Enzyme percolation method can significantly increase the extraction rate of the main active substance-hyoscyamine in beauty herbal.This method can be used to extract the active substance in beauty herbal.
