CAJ | 학술논문

目的：探讨体外沉默葡萄糖调节蛋白GRP78／BIP表达对膀胱癌T24细胞侵袭及迁移的影响及可能机制。方法：设计、合成靶向GRP78基因的小干扰RNA，利用脂质体Liopfecta mineRNAIMAX转染入膀胱癌T24细胞，分别采用Real-time PCR、Western blot在mRNA和蛋白水平检测细胞内GRP78、MMP2、MMP9、Timp-2表达，采用Transwell实验及细胞划痕实验检测沉默GRP78表达对膀胱癌T24细胞侵袭及迁移的影响。结果：实验（ siRNA-GRP78） T24细胞中GRP78表达显著降低，细胞侵袭迁移能力明显受到抑制，MMP2、MMP9表达降低，而Timp-2表达增高，差异均有统计学意义（ P＜0.05）。结论：沉默GRP78能明显抑制人膀胱癌细胞的侵袭、迁移能力，其机制可能与影响MMP2、MMP9、Timp-2表达相关。
목적：탐토체외침묵포도당조절단백GRP78／BIP표체대방광암T24세포침습급천이적영향급가능궤제。방법：설계、합성파향GRP78기인적소간우RNA，이용지질체Liopfecta mineRNAIMAX전염입방광암T24세포，분별채용Real-time PCR、Western blot재mRNA화단백수평검측세포내GRP78、MMP2、MMP9、Timp-2표체，채용Transwell실험급세포화흔실험검측침묵GRP78표체대방광암T24세포침습급천이적영향。결과：실험（ siRNA-GRP78） T24세포중GRP78표체현저강저，세포침습천이능력명현수도억제，MMP2、MMP9표체강저，이Timp-2표체증고，차이균유통계학의의（ P＜0.05）。결론：침묵GRP78능명현억제인방광암세포적침습、천이능력，기궤제가능여영향MMP2、MMP9、Timp-2표체상관。
Objective:To investigate effect of silencing GRP78 in the invasion and metastasis of Bladder cancer T24 cells and the possible mechanism.Methods: The siRNA targeting GRP78 gene was chemically synthesized, and transfect into human bladder cancer T24 cells by Liopfecta mineRNAIMAX.The mRNA and protein expression levels of GRP78,MMP2,MMP9 and Timp-2 were detected by Real-time PCR and Western blot.Transwell assay and Scratch Wound Assay were conducted to deter mine the potency of migration and invasion of T24 cells.Results:The expression of AEG1 mRNA and protein were significantly down-regulated in T24 cells transfected with siRNA targeting GRP78 as compared with the control group( P<0.01);so was expression levels of MMP2 and MMP9 protein( P<0.05 );but timp-2 expression was up-regulated.Cell migration and invasion capacity of T24 cells tranfected with GRP78siRNA group had lower than the cells in the transfection with siRNA-control group(P<0.05).Conclusion:GRP78 functions as a positive regulator in the invasion and metastasis of bladder cancer cells,and may be involved in the expression of MMP2,MMP9 and Tipm-2.