中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
Chinese Journal of Immunology
2015年
11期
1486-1489
,共4页
亥茅酚苷%巨噬细胞%炎症%一氧化氮%白介素6
亥茅酚苷%巨噬細胞%炎癥%一氧化氮%白介素6
해모분감%거서세포%염증%일양화담%백개소6
Sec-O-Glucosylhamaudol%Macrophage%Inflammation%Nitric oxide%Interleukin-6
目的：探讨亥茅酚苷对脂多糖（ LPS）诱导的小鼠巨噬细胞株RAW264.7炎症介质及细胞因子（ NO、IL-6）释放的影响。方法：应用LPS（1μg／ml）刺激RAW264.7细胞，采用不同浓度亥茅酚苷（20、40、80μg／ml）干预，Griess试剂法测定NO释放量；酶联免疫吸附试验（ ELISA）测定IL-6释放，用免疫印迹法（ Western blot）检测细胞一氧化氮合酶（ iNOS）的表达，实时荧光定量反转录聚合酶链反应（ RT-PCR ）技术分析iNOS、IL-6 mRNA的表达。结果：亥茅酚苷各剂量组（20、40、80μg／ml）均能抑制LPS诱导的RAW264.7细胞NO、IL-6的释放（P＜0.01），并下调iNOS蛋白、iNOS mRNA、IL-6 mRNA的表达。结论：亥茅酚苷对LPS诱导的巨噬细胞NO、IL-6释放和iNOS表达有抑制作用，具有一定的抗炎活性。
目的：探討亥茅酚苷對脂多糖（ LPS）誘導的小鼠巨噬細胞株RAW264.7炎癥介質及細胞因子（ NO、IL-6）釋放的影響。方法：應用LPS（1μg／ml）刺激RAW264.7細胞，採用不同濃度亥茅酚苷（20、40、80μg／ml）榦預，Griess試劑法測定NO釋放量；酶聯免疫吸附試驗（ ELISA）測定IL-6釋放，用免疫印跡法（ Western blot）檢測細胞一氧化氮閤酶（ iNOS）的錶達，實時熒光定量反轉錄聚閤酶鏈反應（ RT-PCR ）技術分析iNOS、IL-6 mRNA的錶達。結果：亥茅酚苷各劑量組（20、40、80μg／ml）均能抑製LPS誘導的RAW264.7細胞NO、IL-6的釋放（P＜0.01），併下調iNOS蛋白、iNOS mRNA、IL-6 mRNA的錶達。結論：亥茅酚苷對LPS誘導的巨噬細胞NO、IL-6釋放和iNOS錶達有抑製作用，具有一定的抗炎活性。
목적：탐토해모분감대지다당（ LPS）유도적소서거서세포주RAW264.7염증개질급세포인자（ NO、IL-6）석방적영향。방법：응용LPS（1μg／ml）자격RAW264.7세포，채용불동농도해모분감（20、40、80μg／ml）간예，Griess시제법측정NO석방량；매련면역흡부시험（ ELISA）측정IL-6석방，용면역인적법（ Western blot）검측세포일양화담합매（ iNOS）적표체，실시형광정량반전록취합매련반응（ RT-PCR ）기술분석iNOS、IL-6 mRNA적표체。결과：해모분감각제량조（20、40、80μg／ml）균능억제LPS유도적RAW264.7세포NO、IL-6적석방（P＜0.01），병하조iNOS단백、iNOS mRNA、IL-6 mRNA적표체。결론：해모분감대LPS유도적거서세포NO、IL-6석방화iNOS표체유억제작용，구유일정적항염활성。
Objective:To investigate the effect of Sec-O-Glucosylhamaudol on inflammatory mediator and cytokine( NO and IL-6)production in Lipopolysaccharide(LPS) stimulated murine macrophage(RAW264.7).Methods: Macrophages were induced with LPS,and incubated with different concentrations of Sec-O-Glucosylhamaudol(20,40,80 μg/ml),the quantity of NO production was measured by Griess reagent;the IL-6 production were measured by enzyme linked immunosorbent assay(ELISA),the expression of nitric oxide synthase( iNOS) in cells were detected by Western blot;the expression of iNOS and IL-6 mRNA were analyzed by real-time PCR.Results:Each concentrations of Sec-O-Glucosylhamaudol(20,40,80 μg/ml) inhibited the production of NO and IL-6 in LPS-stimulated RAW264.7 cells(P<0.01).This compound also reduced the mRNA expression of iNOS and IL-6.Conclusion:Sec-O-Glu-cosylhamaudol exhibited anti-inflammatory activity by inhibited the NO and IL-6 production in LPS stimulated RAW264.7 cells.
