CAJ | 학술논문

目的：选取毒力因子多、毒性强的嗜水气单胞菌株（ Aeromonas hydrophil）制成灭活疫苗，研究疫苗的免疫保护效果。方法：采用甲醛灭活制备疫苗，对银鲫进行腹腔注射，设立免疫组和对照组，然后进行血清抗体效价检测、病理切片分析和攻毒保护试验。结果：银鲫在经注射免疫后，各免疫时间均有抗体产生，抗体效价在第6周检测时达到高峰，而对照组在整个试验过程中均没有检测到抗体；病理切片也表明，该疫苗能够对鲫鱼靶器官产生很好的保护作用；攻毒保护试验中，免疫组的免疫保护率达100％，且免疫保护期长达6个月以上。结论：嗜水气单胞菌灭活疫苗对银鲫有显著的免疫保护效应，可作为预防细菌性败血症感染的疫苗。
목적：선취독력인자다、독성강적기수기단포균주（ Aeromonas hydrophil）제성멸활역묘，연구역묘적면역보호효과。방법：채용갑철멸활제비역묘，대은즉진행복강주사，설립면역조화대조조，연후진행혈청항체효개검측、병리절편분석화공독보호시험。결과：은즉재경주사면역후，각면역시간균유항체산생，항체효개재제6주검측시체도고봉，이대조조재정개시험과정중균몰유검측도항체；병리절편야표명，해역묘능구대즉어파기관산생흔호적보호작용；공독보호시험중，면역조적면역보호솔체100％，차면역보호기장체6개월이상。결론：기수기단포균멸활역묘대은즉유현저적면역보호효응，가작위예방세균성패혈증감염적역묘。
Objective:Selected virulence factors more than and high virulent Aeromonas hydrophia strain made into inactivated vaccine,to study the immunization effect of inactivated vaccine.Methods:Crucian were vaccinated with formalin-killed vaccine via in-traperitoneal injection.Controls were injected with the same volumes of saline.Then the antibody titres, histopathology and relative percent survival were analyzed from samples of both groups.Results: The antibody in the indirect agglutination reaction could be detected in vaccinated fish once a weeks after immunization and reached highest level 6 weeks after immunization.The histopathology analysis indicated that the vaccine had a good protective effect on crucian target organs.Vaccinated fish showed 100%relative percent survival and the immune period would be 6 month.Conclusion:The vaccine in this study has a significant protective effect on crucian and may be used as effective fish vaccines against bacterial septicemia.