CAJ | 학술논문

目的：：用氚-标记胸腺嘧啶核苷(3 H-TdR )掺入法观察转化生长因子β1( TGF-β1)对人视网膜色素上皮细胞( retinal pigment epithelium cells,RPE)增殖的影响,探讨TGF-β1和RPE细胞在增殖性玻璃体视网膜病变发病机制中的作用。方法：RPE细胞体外培养,用不同浓度的TGF-β10.1、1.0、5.0、10.0和100.0μg/L对细胞进行处理,3 H-TdR掺入法测定细胞放射性强度。结果：TGF-β10.1、1.0、5.0、10.0μg/L 作用后 RPE 活细胞数增加,细胞3H-TdR 摄入量均较对照组显著增加,TGF-β1100.0μg/L对RPE作用相反,细胞3H-TdR摄入量明显低于对照组(P<0.01)。结论：TGF-β1对人RPE细胞增殖有双相调节性,其不同作用的发挥依赖TGF-β1的浓度,其中TGF-β1促RPE细胞增殖作用是诱导增殖性玻璃体视网膜病变发生的可能机制之一。
목적：：용천-표기흉선밀정핵감(3 H-TdR )참입법관찰전화생장인자β1( TGF-β1)대인시망막색소상피세포( retinal pigment epithelium cells,RPE)증식적영향,탐토TGF-β1화RPE세포재증식성파리체시망막병변발병궤제중적작용。방법：RPE세포체외배양,용불동농도적TGF-β10.1、1.0、5.0、10.0화100.0μg/L대세포진행처리,3 H-TdR참입법측정세포방사성강도。결과：TGF-β10.1、1.0、5.0、10.0μg/L 작용후 RPE 활세포수증가,세포3H-TdR 섭입량균교대조조현저증가,TGF-β1100.0μg/L대RPE작용상반,세포3H-TdR섭입량명현저우대조조(P<0.01)。결론：TGF-β1대인RPE세포증식유쌍상조절성,기불동작용적발휘의뢰TGF-β1적농도,기중TGF-β1촉RPE세포증식작용시유도증식성파리체시망막병변발생적가능궤제지일。
Objective:To investigate the effects of transforming growth factorβ1 ( TGF-β1 ) on the proliferation of human retinal pigment epithelium( RPE ) cells by 3 H- thymidine deoxyribose ( 3 H-TdR ) absorption, and explore the pathogenesis mechanism of vitreoretinopathy. Methods:Human retinal pigment epithelium cells were cultured in vitro,the cells were incubated with 0. 1,1. 0,5. 0, 10. 0 and 100. 0 μg/L of TGF-β1 . The radioactivity intensity of cells were determined by 3 H-TdR absorption. Results:The 0. 1,1. 0,5. 0 and 10. 0 μg/L of TGF-β1 could promote the proliferation of RPE cells to increase the number of living cells,the intaking 3 H-TdR value of cells was significantly higher than that in control group(P <0. 01 ). The effects of the 100. 0 μg/L of TGF-β1 on the proliferation of RPE cells was opposite,the intaking 3H-TdR value of cells was significantly lower than that in control group(P<0. 01). Conclusions:The effect of TGF-β1 on the proliferation of RPE cells is dual regulation, different concentrations of TGF-β1 may have different effects on RPE cells. The effect of TGF-β1 on promoting the proliferation of RPE cells maybe one of mechanisms of proliferative vitreoretinopathy.