CAJ | 학술논문

目的：利用降植烷构建一种模拟人类风湿性关节炎发病过程的小鼠模型。方法：6～8周雌性BALB／c小鼠30只，随机分为模型组和对照组，每组15只。模型组于0周、9周、18周时经腹腔注射0.5 ml降植烷；对照组注射0.5 ml生理盐水。观察各组小鼠后足足掌厚度变化，并对关节炎的肿胀程度进行评分；H＆E染色评估滑膜增生和炎性细胞浸润程度；流式细胞术方法检测小鼠脾脏中巨噬细胞、树突状细胞、中性粒细胞、T细胞和B细胞的亚群变化。结果：21周时，模型组有11只小鼠出现足爪红肿，足掌平均厚度为（2.90±0.51）mm，与对照组（1.29±0.47）mm比较，差异有统计学意义（P＜0.05）；关节炎评分为9.55±2.80。 H＆E染色结果显示模型组胫骨远端骨小梁吸收破坏，累及骨皮质，关节软骨可见坏死脱落、纤维化，呈现出明显的类风湿关节炎病理改变。流式细胞术分析结果显示，造模21周时，脾脏细胞中CD11b、CD11c和GR1表达明显增加，提示巨噬细胞、树突状细胞和中性粒细胞亚群数量增加；同时，CD4、CD8、CD154的分子表达也明显上调，与对照组比较，差异有统计学意义（ P＜0.05）。结论：经腹腔注射降植烷能够成功诱导小鼠关节炎，并产生与RA患者类似的组织病理和免疫学改变。降植烷诱导的小鼠关节炎模型，能够较为准确地模拟临床类风湿关节炎的发病过程。
목적：이용강식완구건일충모의인류풍습성관절염발병과정적소서모형。방법：6～8주자성BALB／c소서30지，수궤분위모형조화대조조，매조15지。모형조우0주、9주、18주시경복강주사0.5 ml강식완；대조조주사0.5 ml생리염수。관찰각조소서후족족장후도변화，병대관절염적종창정도진행평분；H＆E염색평고활막증생화염성세포침윤정도；류식세포술방법검측소서비장중거서세포、수돌상세포、중성립세포、T세포화B세포적아군변화。결과：21주시，모형조유11지소서출현족조홍종，족장평균후도위（2.90±0.51）mm，여대조조（1.29±0.47）mm비교，차이유통계학의의（P＜0.05）；관절염평분위9.55±2.80。 H＆E염색결과현시모형조경골원단골소량흡수파배，루급골피질，관절연골가견배사탈락、섬유화，정현출명현적류풍습관절염병리개변。류식세포술분석결과현시，조모21주시，비장세포중CD11b、CD11c화GR1표체명현증가，제시거서세포、수돌상세포화중성립세포아군수량증가；동시，CD4、CD8、CD154적분자표체야명현상조，여대조조비교，차이유통계학의의（ P＜0.05）。결론：경복강주사강식완능구성공유도소서관절염，병산생여RA환자유사적조직병리화면역학개변。강식완유도적소서관절염모형，능구교위준학지모의림상류풍습관절염적발병과정。
Objective:To establish a pristine-induced rheumatoid arthritis model in mice,and to evaluate its histological and immunological distinction.Methods:Thirty female BALB/c mice,6-8 weeks old,were randomly divided into 2 groups,a control group and pristine group.The mice in pristine group were injected intraperitoneally with 0.5 ml pristine three times at 0,9,and 18 weeks, while mice in the control group receiving saline at the same time.Arthritis score and paw thickness were measured and histopathological assessment of joint sections was performed.The expression of phagocytes,dendritic,neutrophils,T and B cells markers in spleen were determined by flow cytometry.Results:In model-marking group,11 mice were presented with macroscopic evidence of arthritis such as erythema or swelling.The paw thickness in pristine-induced mice was significant higher than that in the control groups[(2.90±0.51) mm vs(1.29±0.47 mm),P<0.05].In addition,arthritis score in pristine-induced mice was 9.55±2.80 at 21 weeks after first injection with 0.5 ml pristine.H&E staining revealed a significant increase of synovial inflammation, cartilage and bone destruction after stimulated with pristine.Meanwhile,the expression levels of CD11b,CD11c,GR1,CD4,CD8 and CD154 were obviously increased in model-marking group when compared with that in control group.Conclusion: The pristine-induced model presents the similar histological and immunological distinctions with human rheumatism arthritis,which can mimic the pathogenesis of rheumatism arthritis.