CAJ | 학술논문

目的：果糖溶液喂养 Wistar 雄性大鼠，注射 Wistar 大鼠不同浓度的鞣云实素检测大鼠体内胰岛淀粉样多肽（IAPP）、胰高血糖素样肽-1（GLP -1）及胰岛素（INS）浓度的变化。方法 Wistar 大鼠给予相同浓度果糖溶液连续灌胃3周，第4周腹腔注射不同浓度的鞣云实素溶液，隔1 d 注射1次，连续注射3次，第7天取大鼠血清，利用酶标仪测定血清中胰岛淀粉样多肽、胰高血糖素样肽-1及胰岛素的浓度。结果果糖溶液喂养后胰岛素分泌减少、胰岛淀粉样多肽浓度降低、胰高血糖素样肽-1浓度升高；注射20 mg·kg -1鞣云实素溶液后胰岛素分泌增多、胰岛淀粉样多肽浓度明显降低、胰高血糖素样肽-1浓度明显增高；注射40 mg·kg -1鞣云实素溶液后胰岛素分泌与对照组相比明显减少、胰岛淀粉样多肽浓度明显增高且高于对照组、胰高血糖素样肽-1浓度明显降低。结论低浓度鞣云实素能够促进胰高血糖素样肽-1生成、刺激胰岛素分泌及抑制胰岛淀粉样多肽的形成，本实验为进一步研究鞣云实素对胰岛素抵抗大鼠模型的相关激素变化提供一定基础。
목적：과당용액위양 Wistar 웅성대서，주사 Wistar 대서불동농도적유운실소검측대서체내이도정분양다태（IAPP）、이고혈당소양태-1（GLP -1）급이도소（INS）농도적변화。방법 Wistar 대서급여상동농도과당용액련속관위3주，제4주복강주사불동농도적유운실소용액，격1 d 주사1차，련속주사3차，제7천취대서혈청，이용매표의측정혈청중이도정분양다태、이고혈당소양태-1급이도소적농도。결과과당용액위양후이도소분비감소、이도정분양다태농도강저、이고혈당소양태-1농도승고；주사20 mg·kg -1유운실소용액후이도소분비증다、이도정분양다태농도명현강저、이고혈당소양태-1농도명현증고；주사40 mg·kg -1유운실소용액후이도소분비여대조조상비명현감소、이도정분양다태농도명현증고차고우대조조、이고혈당소양태-1농도명현강저。결론저농도유운실소능구촉진이고혈당소양태-1생성、자격이도소분비급억제이도정분양다태적형성，본실험위진일보연구유운실소대이도소저항대서모형적상관격소변화제공일정기출。
Objective To feed male Wistar rats with fructose solution,and inject different concentration of corilagin so-lution to Wistar rats,determine the changes of the concentration of IAPP,GLP - 1)and INS in rats serum. Methods To give same concentration of fructose solution to Wistar rats three weeks by intragastric administration. In the fourth week,dif-ferent concentration of corilagin solution was admined by intraperitoneal injection,once every two days and three times. In the seventh day,blood serum of rats was collected and the concentrations of IAPP,GLP - 1 and INS were determined by ELISA. Results After fed fructose solution,the rats secreted insulin was decreased,the concentration of IAPP was reduced and the concentration of GLP - 1 was increased;After injected the concentration of 20 mg·kg - 1 corilagin,the insulin secre-tion was increased,the concentration of IAPP was decreased significantly,the concentration of GLP - 1 was increased signifi-cantly;When injected the concentration of 40 mg·kg - 1 corilagin,the insulin secretion was decreased significantly to com-pared with control group,the concentration of IAPP was increased significantly and higher than control group,the concentra-tion of GLP - 1 was reduced significantly and lower than control group. Conclusion Corilagin at low concentration can pro-mote the generation of GLP - 1,stimulate insulin secretion and inhibit the formation of IAPP. The experiment provided some basis on the further research of corilagin for the hormonal changes related to insulin resistance in rats model.