CAJ | 학술논문

目的：：检测肝脏核因子( NF)-κB的表达,评估γ-干扰素( IFN-γ)对乳鼠感染轮状病毒( RV)后的作用。方法：将乳鼠分为肠道外感染对照组、IFN-γ大小剂量干预组。腹腔注射RV建立2日龄RV肠道外感染模型。用大小剂量(2μg,0.2μg) IFN-γ在BALB/C乳鼠背部皮下注射3 d。模型建立后第5天取材,观察肝脏病理组织学变化,蛋白质印迹法和免疫组织化学法检测肝脏NF-κB表达。结果：IFN-γ小剂量干预组乳鼠肝脏病理损伤评分较肠道外感染对照组差异无统计学意义( P>0.05)。 IFN-γ大剂量干预组乳鼠肝脏病理损伤评分均较肠道外感染对照组和IFN-γ小剂量干预组明显增高(P<0.01)。蛋白质印迹法和免疫组织化学法显示IFN-γ大小剂量干预组NF-κB表达随IFN-γ剂量的增加而增加(P<0.01)。结论：IFN-γ能刺激NF-κB的表达。肝脏的病理损伤与NF-κB在肝脏的表达量相关。
목적：：검측간장핵인자( NF)-κB적표체,평고γ-간우소( IFN-γ)대유서감염륜상병독( RV)후적작용。방법：장유서분위장도외감염대조조、IFN-γ대소제량간예조。복강주사RV건립2일령RV장도외감염모형。용대소제량(2μg,0.2μg) IFN-γ재BALB/C유서배부피하주사3 d。모형건립후제5천취재,관찰간장병리조직학변화,단백질인적법화면역조직화학법검측간장NF-κB표체。결과：IFN-γ소제량간예조유서간장병리손상평분교장도외감염대조조차이무통계학의의( P>0.05)。 IFN-γ대제량간예조유서간장병리손상평분균교장도외감염대조조화IFN-γ소제량간예조명현증고(P<0.01)。단백질인적법화면역조직화학법현시IFN-γ대소제량간예조NF-κB표체수IFN-γ제량적증가이증가(P<0.01)。결론：IFN-γ능자격NF-κB적표체。간장적병리손상여NF-κB재간장적표체량상관。
Objective:To explore the expression of hepatic NF-κB for evaluating the effects of IFN-γon suckling mouse with rotavirus ( RV) infection. Methods:The suckling mouse were divided into the control group,high and low-dose IFN-γgroups. The extraintestinal rotavirus infection model of 2-day-old suckling mouse was established by intraperitoneal injection of rotavirus. The 2 μg and 0. 2 μg IFN-γ were injected subcutaneously into the back of BALB/C suckling mouse for 3 days. The pathological changes and expressions of NF-κB detected by Western blot and immunohistochemistry in liver were observed after 5 days of model establishment. Results:The difference of the score of liver pathological damage between low-dose IFN-γgroup and control group was not statistically significant( P>0. 05). The score of liver pathological damage in high-dose IFN-γgroup was significantly higher than that in control group and low-dose IFN-γ group(P<0. 01). Western blotting and immunohistochemistry results showed that the expression of NF-κB increased with the dose increasing of IFN-γ(P<0. 01). Conclusions:IFN-γ can stimulate the expression of NF-κB. The pathological damage of the liver was correlated with the expression of NF-κB.