广州化工
廣州化工
엄주화공
Guangzhou Chemical Industry
2015年
22期
120-123,185
,共5页
清火片%HPLC%含量测定
清火片%HPLC%含量測定
청화편%HPLC%함량측정
Qinghuo Pian%HPLC%content determination
建立了清火片含量测定的方法。对大黄药材中大黄素和大黄酚提取方法进行实验对比研究，利用HPLC法进行含量测定。结果表明：大黄素浓度在2.56~160μg/mL范围内吸收度成良好线性关系( R2=0.9999)，平均回收率为107.1%， RSD为1.40%；大黄酚浓度在1.72~34.4μg/mL 范围内吸收度成良好线性关系( R2=0.9997)，平均回收率为100.91%， RSD 为0.82%。 HPLC法进行含量测定方法简便、准确可靠，可用于清火片的含量测定。
建立瞭清火片含量測定的方法。對大黃藥材中大黃素和大黃酚提取方法進行實驗對比研究，利用HPLC法進行含量測定。結果錶明：大黃素濃度在2.56~160μg/mL範圍內吸收度成良好線性關繫( R2=0.9999)，平均迴收率為107.1%， RSD為1.40%；大黃酚濃度在1.72~34.4μg/mL 範圍內吸收度成良好線性關繫( R2=0.9997)，平均迴收率為100.91%， RSD 為0.82%。 HPLC法進行含量測定方法簡便、準確可靠，可用于清火片的含量測定。
건립료청화편함량측정적방법。대대황약재중대황소화대황분제취방법진행실험대비연구，이용HPLC법진행함량측정。결과표명：대황소농도재2.56~160μg/mL범위내흡수도성량호선성관계( R2=0.9999)，평균회수솔위107.1%， RSD위1.40%；대황분농도재1.72~34.4μg/mL 범위내흡수도성량호선성관계( R2=0.9997)，평균회수솔위100.91%， RSD 위0.82%。 HPLC법진행함량측정방법간편、준학가고，가용우청화편적함량측정。
Content determination of Qinghuo Pian was established. Emodin and rhubarb phenol extraction in rhubarb medicinal materials were discussed, content using HPLC method for determinate. Results showed that concentration of emodin was up to 2. 56~160 μg/mL with a good linear relationship with absorbance ( R2=0. 9999 ) , the average recovery was 107. 1%, RSD was 1. 40%. Concentration of rhubarb phenol was up to 1. 72 to 34. 4 μg/mL with a good linear relationship with absorbance ( R2 = 0. 9997 ) , the average recovery was 100. 91%, RSD was 0. 82%. HPLC method for content determination method is simple, accurate, reliable and can be used for content determination of Qinghuo Pian.
