中华医学美学美容杂志
中華醫學美學美容雜誌
중화의학미학미용잡지
Chinese Journal of Medical Aesthetics and Cosmetology
2015年
5期
300-303
,共4页
脂肪组织%生长因子%血管生成
脂肪組織%生長因子%血管生成
지방조직%생장인자%혈관생성
Adipose tissue%Growth factor%Angiogenesis
目的探讨优化血管内皮生长因子165 (VEGF165)、血管生成素-1(Ang-1)、碱性成纤维细胞生长因子(bFGF)的配比对移植脂肪颗粒血管生成的影响.方法将18只大鼠分成9组,每组2只,9组的VEGF165、Ang-1、bFGF配比依次为1∶0∶0、0∶1∶0、0∶0∶1、1/2∶1/2∶0、0∶1/2∶1/2、1/2∶0∶1/2、1/3∶1/3∶1/3、2/3∶1/6∶1/6、1/6∶2/3∶1/6;将9组配比溶液分别注入9组大鼠自体颗粒脂肪中,移植于每只大鼠背部4个受区,术后15周HE染色观察脂肪细胞形态,免疫组织化学得到毛细血管数目,用Scheffe模型进行血管生成的最优生长因子配比.结果 1种生长因子组脂肪空泡大小不均,2种生长因子组脂肪细胞大小趋于均一,3种生长因子组脂肪细胞大小均一,接近正常形态;Scheffe模型优化最大血管数目为12.09点,VEGF165、Ang-1、bFGF配比为0.380∶0.280∶0.340.结论 当VEGF165、Ang-1、bFGF配比为0.380∶0.280∶0.340时,移植脂肪内新生血管密度最高,生长因子促移植脂肪成活作用最强.
目的探討優化血管內皮生長因子165 (VEGF165)、血管生成素-1(Ang-1)、堿性成纖維細胞生長因子(bFGF)的配比對移植脂肪顆粒血管生成的影響.方法將18隻大鼠分成9組,每組2隻,9組的VEGF165、Ang-1、bFGF配比依次為1∶0∶0、0∶1∶0、0∶0∶1、1/2∶1/2∶0、0∶1/2∶1/2、1/2∶0∶1/2、1/3∶1/3∶1/3、2/3∶1/6∶1/6、1/6∶2/3∶1/6;將9組配比溶液分彆註入9組大鼠自體顆粒脂肪中,移植于每隻大鼠揹部4箇受區,術後15週HE染色觀察脂肪細胞形態,免疫組織化學得到毛細血管數目,用Scheffe模型進行血管生成的最優生長因子配比.結果 1種生長因子組脂肪空泡大小不均,2種生長因子組脂肪細胞大小趨于均一,3種生長因子組脂肪細胞大小均一,接近正常形態;Scheffe模型優化最大血管數目為12.09點,VEGF165、Ang-1、bFGF配比為0.380∶0.280∶0.340.結論 噹VEGF165、Ang-1、bFGF配比為0.380∶0.280∶0.340時,移植脂肪內新生血管密度最高,生長因子促移植脂肪成活作用最彊.
목적탐토우화혈관내피생장인자165 (VEGF165)、혈관생성소-1(Ang-1)、감성성섬유세포생장인자(bFGF)적배비대이식지방과립혈관생성적영향.방법장18지대서분성9조,매조2지,9조적VEGF165、Ang-1、bFGF배비의차위1∶0∶0、0∶1∶0、0∶0∶1、1/2∶1/2∶0、0∶1/2∶1/2、1/2∶0∶1/2、1/3∶1/3∶1/3、2/3∶1/6∶1/6、1/6∶2/3∶1/6;장9조배비용액분별주입9조대서자체과립지방중,이식우매지대서배부4개수구,술후15주HE염색관찰지방세포형태,면역조직화학득도모세혈관수목,용Scheffe모형진행혈관생성적최우생장인자배비.결과 1충생장인자조지방공포대소불균,2충생장인자조지방세포대소추우균일,3충생장인자조지방세포대소균일,접근정상형태;Scheffe모형우화최대혈관수목위12.09점,VEGF165、Ang-1、bFGF배비위0.380∶0.280∶0.340.결론 당VEGF165、Ang-1、bFGF배비위0.380∶0.280∶0.340시,이식지방내신생혈관밀도최고,생장인자촉이식지방성활작용최강.
Objective To explore the optimal formula of vascular endothelial growth factor,angiopoietin and basic fibroblast growth factor on autologous fat angiogenesis and tissue proliferation.Methods Wistar rat mesenteric fat in 9 groups trimmed into particles were added a 6μl complex solution of the three factors in the formula of 1 ∶ 0 ∶ 0,0 ∶ 1 ∶ 0,0 ∶ 0 ∶ 1,1/2 ∶ 1/2 ∶ 0,0 ∶ 1/2 ∶ 1/2,1/2 ∶ 0 ∶ 1/2,1/3 ∶ 1/3 ∶ 1/3,2/3 ∶ 1/6 ∶ 1/6,1/6 ∶ 2/3 ∶ 1/6,then injected in each rat subcutaneously.15 weeks later the transplantated fat was removed,to observe the fat cell appearance and counting the number of capillaries in the graft.Statistical genetic algorithm was used to set up a Scheffer egression model to optimize fat angiogenesis number.Results In one growth factor group fat vacuoles was uneven size;in two growth factors group,fat cell size tended to be homogeneous and in the three growth factor group,fat cell size was uniformity;the highest angiogenesis rate was 12.092,when the formula of VEGF165,Ang-1 and b-FGF was 0.380∶0.280:0.340.Conclusions Formula of VEGF165,Ang-1,bFGF at 0.380 ∶ 0.280 ∶ 0.340 could improve blood vessels density and increase implanted fat survival.
