CAJ | 학술논문

真菌等临床病原体的诊断、基因分型和耐药性检测对于临床诊治十分重要. 多重连接探针扩增是一个可以同时定量检测多个基因序列的,简单、有效、快速的方法. 其步骤包括待测DNA变性、半探针与待测DNA杂交,半探针的连接、PCR法扩增、凝胶电泳或者毛细管电泳鉴定扩增产物. MLPA已经在临床微生物诊断和研究中有了一定的应用,虽然尚处于起步阶段,但因其操作简便,反应快速,高通量,高敏感性和特异性的特点,拥有良好的应用前景.
진균등림상병원체적진단、기인분형화내약성검측대우림상진치십분중요. 다중련접탐침확증시일개가이동시정량검측다개기인서렬적,간단、유효、쾌속적방법. 기보취포괄대측DNA변성、반탐침여대측DNA잡교,반탐침적련접、PCR법확증、응효전영혹자모세관전영감정확증산물. MLPA이경재림상미생물진단화연구중유료일정적응용,수연상처우기보계단,단인기조작간편,반응쾌속,고통량,고민감성화특이성적특점,옹유량호적응용전경.
As for patients diagnosed with Infectious diseases,favorable prognosis is determined by correct diagnosis and proper treatment.So it is important to diagnose clinical pathogens rapidly.Compared with other clinical test methods,MLPA is a simple,val?id,and fast one which can detect several gene sequences in quantity at the same time. The steps include:the degeneration of DNA sample,hybridization,ligation,and PCR amplification.Although being at its first stage,there are already certain applications of MLPA in diagnosing and researching of clinic microorganism,and the peculiarity of easy operation,fast reaction,high pass rate and high sen?sitivity have reveal bright prospect of MLPA.