CAJ | 학술논문

目的核酸序列依赖性扩增 ( nucleic acid sequence?based amplification,NASBA)、Real?time PCR及GM试验在侵袭性曲霉菌感染中的诊断价值. 方法收集2013年11月~2014年6月临床上曲霉菌感染高危病患的血液标本80例,并根据EORTC/MSG诊断标准分为确诊组8例,拟诊组26例,非感染组46例,分别利用NASBA、real?time PCR及GM试验进行检测,计算3种方法的诊断指标并分析评价. 结果 NASBA、real?time PCR及GM试验3种方法的灵敏度分别为76.47%、67.65%、52.94%,特异度分别为80.43%、89.13%、80.43%. 联合诊断结果显示,NASBA与real?time PCR串联方案有最好的特异度 (100%)及阳性预测值(100%);NASBA与real?time PCR并联方案则最为灵敏(94.12%). 结论 NASBA用于诊断IA最为敏感,而real?time PCR则最为特异,GM试验的表现差于前两者. 联合应用的诊断策略,在特定情况下提高临床早期诊断IA的准确性.
목적 핵산서렬의뢰성확증 ( nucleic acid sequence?based amplification,NASBA)、Real?time PCR급GM시험재침습성곡매균감염중적진단개치. 방법 수집2013년11월~2014년6월림상상곡매균감염고위병환적혈액표본80례,병근거EORTC/MSG진단표준분위학진조8례,의진조26례,비감염조46례,분별이용NASBA、real?time PCR급GM시험진행검측,계산3충방법적진단지표병분석평개. 결과 NASBA、real?time PCR급GM시험3충방법적령민도분별위76.47%、67.65%、52.94%,특이도분별위80.43%、89.13%、80.43%. 연합진단결과현시,NASBA여real?time PCR천련방안유최호적특이도 (100%)급양성예측치(100%);NASBA여real?time PCR병련방안칙최위령민(94.12%). 결론 NASBA용우진단IA최위민감,이real?time PCR칙최위특이,GM시험적표현차우전량자. 연합응용적진단책략,재특정정황하제고림상조기진단IA적준학성.
Objective To study the diagnostic performance of nucleic acid sequence?based amplification ( NASBA) assay,real?time PCR and GM test in detecting invasive aspergillosis for clinical diagnosis.Methods Blood samples from 80 patients at a high risk for IA were collected during from November 2013 to June 2014.These patients were categorized as 8 proven IA,26 probable IA, and 46 non?IA according to the 2008 revised definitions of EORTC/MSG.Blood samples were tested by NASBA,real?time PCR and GM test and their diagnostic parameters were calculated,respectively.Result The sensitivity of NASBA,real?time PCR and GM test was 76.47%,67.65% and 52.94%,while their specificity was 80.43%,89.13%,80.43%,respectively.The efficiency of various com?binations of tests was also evaluated.Perfect specificity (100%) and positive predictive value (100%) were achieved by combining NASBA and real?time PCR as a serial testing.A combination of NASBA and real?time PCR as a parallel testing was the most sensitive (94.12%).Conclusion The sensitivity and specificity of NASBA and real?time PCR were superior to GM test.Combination of these assays could be particularly useful in specific clinical situations.