中国真菌学杂志
中國真菌學雜誌
중국진균학잡지
Chinese Journal of Mycology
2015年
5期
266-271
,共6页
王瑞丽%敖俊红%廖勇%杨蓉娅%杨冬倩%吕雪莲%陈珊珊
王瑞麗%敖俊紅%廖勇%楊蓉婭%楊鼕倩%呂雪蓮%陳珊珊
왕서려%오준홍%료용%양용아%양동천%려설련%진산산
阿萨希毛孢子菌%树突状细胞%表面分子
阿薩希毛孢子菌%樹突狀細胞%錶麵分子
아살희모포자균%수돌상세포%표면분자
T.asahii%dendritic cells%surface molecules
目的 通过体外研究热灭活阿萨希毛孢子菌 (Trichosporon asahii,T.asahii)孢子对健康人外周血单核源树突状细胞(dendritic cells,DCs)的表型和功能的影响,探讨DCs在T.asahii感染中可能发挥的作用. 方法 体外诱导培养正常人外周血DCs,与不同浓度的热灭活T.asahii孢子进行孵育,DCs与T.asahii浓度比分别为1 : 1、1 : 5和1 : 10,RPMI?1640及脂多糖 ( lipopolysaccharide,LPS)刺激组分别作为空白对照及阳性对照,在显微镜下观察各组DCs形态的变化;瑞氏?姬姆萨染色观察实验组DCs对T.asahii的吞噬情况并计算吞噬率;流式细胞术检测各组DCs表面共刺激分子表达情况. 结果 诱导孵育过程中DCs形态发生明显改变;24 h时实验组部分DCs内可观察到不止一个被吞噬的T. asahii孢子,吞噬率组间差异有统计学意义 ( P<0.05);与空白对照组相比,实验组DCs形态发生明显改变,且表面共刺激分子CD80、CD86、CD83表达水平明显升高 ( P<0.05) ,但升高水平低于LPS刺激组,差异有统计学意义 ( P<0.05). 结论 人外周血DCs吞噬热灭活的T. asahii孢子后,形态发生改变,表面分子表达水平升高,DCs进一步成熟.
目的 通過體外研究熱滅活阿薩希毛孢子菌 (Trichosporon asahii,T.asahii)孢子對健康人外週血單覈源樹突狀細胞(dendritic cells,DCs)的錶型和功能的影響,探討DCs在T.asahii感染中可能髮揮的作用. 方法 體外誘導培養正常人外週血DCs,與不同濃度的熱滅活T.asahii孢子進行孵育,DCs與T.asahii濃度比分彆為1 : 1、1 : 5和1 : 10,RPMI?1640及脂多糖 ( lipopolysaccharide,LPS)刺激組分彆作為空白對照及暘性對照,在顯微鏡下觀察各組DCs形態的變化;瑞氏?姬姆薩染色觀察實驗組DCs對T.asahii的吞噬情況併計算吞噬率;流式細胞術檢測各組DCs錶麵共刺激分子錶達情況. 結果 誘導孵育過程中DCs形態髮生明顯改變;24 h時實驗組部分DCs內可觀察到不止一箇被吞噬的T. asahii孢子,吞噬率組間差異有統計學意義 ( P<0.05);與空白對照組相比,實驗組DCs形態髮生明顯改變,且錶麵共刺激分子CD80、CD86、CD83錶達水平明顯升高 ( P<0.05) ,但升高水平低于LPS刺激組,差異有統計學意義 ( P<0.05). 結論 人外週血DCs吞噬熱滅活的T. asahii孢子後,形態髮生改變,錶麵分子錶達水平升高,DCs進一步成熟.
목적 통과체외연구열멸활아살희모포자균 (Trichosporon asahii,T.asahii)포자대건강인외주혈단핵원수돌상세포(dendritic cells,DCs)적표형화공능적영향,탐토DCs재T.asahii감염중가능발휘적작용. 방법 체외유도배양정상인외주혈DCs,여불동농도적열멸활T.asahii포자진행부육,DCs여T.asahii농도비분별위1 : 1、1 : 5화1 : 10,RPMI?1640급지다당 ( lipopolysaccharide,LPS)자격조분별작위공백대조급양성대조,재현미경하관찰각조DCs형태적변화;서씨?희모살염색관찰실험조DCs대T.asahii적탄서정황병계산탄서솔;류식세포술검측각조DCs표면공자격분자표체정황. 결과 유도부육과정중DCs형태발생명현개변;24 h시실험조부분DCs내가관찰도불지일개피탄서적T. asahii포자,탄서솔조간차이유통계학의의 ( P<0.05);여공백대조조상비,실험조DCs형태발생명현개변,차표면공자격분자CD80、CD86、CD83표체수평명현승고 ( P<0.05) ,단승고수평저우LPS자격조,차이유통계학의의 ( P<0.05). 결론 인외주혈DCs탄서열멸활적T. asahii포자후,형태발생개변,표면분자표체수평승고,DCs진일보성숙.
Objective To investigate the effects of Trichosporon asahii on the phenotypic and functional maturation of human pe?ripheral blood monocyte?derived dendritic cells (DCs).Methods The monocyte?derived DCs of health donors were obtained by cul?ture in vitro.In the experimental groups,DCs stimulated by different doses of heat?inactived T.asahii conidia (DCs : T.asahii=1 : 1, 1 : 5,1 : 10) ,RPMI?1640 and lipopolysaccharide ( LPS) stimulation groups were as the blank control and positive control,respec?tively.Shapes of the cells were observed under the inverted microscope,the rate of phagocytosis was calculated by Wright Giemsa stai?ning.DCs maturation was analysed by using flow cytometry.Results The morphology of DCs was significantly changed during incuba?tion.At 24 h,more than one T.asahii conidias were efficiently bound and internalized by DCs and the phagocytic rates were signifi?cantly different between the experimental groups (P<0.05).The expressions of CD80,CD86,CD83 in the experimental groups were much higher than the blank control group (P<0.05),but significantly lower than LPS group (P<0.05).Conclusion T.asahii can change the morphology of DCs and promote the expression of surface molecules,which enhance the maturation of the dendritic cells.