中国真菌学杂志
中國真菌學雜誌
중국진균학잡지
Chinese Journal of Mycology
2015年
5期
261-265,278
,共6页
侵袭性鼻窦炎%烟曲霉%大鼠模型
侵襲性鼻竇炎%煙麯黴%大鼠模型
침습성비두염%연곡매%대서모형
invasive rhinosinusitis%Aspergillus fumigatus%rat model
目的 建立稳定且易操作的急性侵袭性真菌性鼻?鼻窦炎大鼠模型,以促进对急性侵袭性真菌性鼻?鼻窦炎的实验研究,指导临床. 方法 将SD大鼠随机分为四组. A组,免疫抑制+右侧鼻腔填塞Merocel海绵条+右侧鼻腔滴入烟曲霉孢子悬液;B组,右侧鼻腔填塞Merocel海绵条+右侧鼻腔滴入烟曲霉孢子悬液;C组,免疫抑制+右侧鼻腔滴入烟曲霉孢子悬液;D组:对照组. A、B、C组大鼠连续3d鼻内滴入烟曲霉孢子悬液,四组大鼠均于第1次滴菌后第4天处死. 取内眦静脉血监测大鼠免疫抑制情况,对鼻部组织行真菌培养和组织病理学观察. 结果 免疫抑制组大鼠血中性粒细胞明显降低,接种烟曲霉当天中性粒细胞计数<0.1×109/L,其余两组变化不明显. 病理结果显示A组90% (9/10)大鼠鼻腔鼻窦组织被烟曲霉侵袭,10% (1/10)肺部可见侵袭;B、C、D组大鼠无鼻部烟曲霉感染,但C组中20% (2/10)大鼠肺部可见烟曲霉侵袭.真菌培养结果显示A组71% ( 5/7)大鼠鼻部组织烟曲霉培养阳性,其他组均为阴性. 结论 单纯对大鼠进行免疫抑制或单纯造成大鼠鼻腔堵塞均不易造成大鼠鼻腔被真菌侵袭;免疫抑制基础上,对大鼠鼻腔填塞Merocel海绵条后再滴入烟曲霉可以成功建立急性侵袭性真菌性鼻?鼻窦炎模型,其成模率高,模型稳定,操作简易,有利于对此疾病在免疫、病理、药物等方面的深入研究.
目的 建立穩定且易操作的急性侵襲性真菌性鼻?鼻竇炎大鼠模型,以促進對急性侵襲性真菌性鼻?鼻竇炎的實驗研究,指導臨床. 方法 將SD大鼠隨機分為四組. A組,免疫抑製+右側鼻腔填塞Merocel海綿條+右側鼻腔滴入煙麯黴孢子懸液;B組,右側鼻腔填塞Merocel海綿條+右側鼻腔滴入煙麯黴孢子懸液;C組,免疫抑製+右側鼻腔滴入煙麯黴孢子懸液;D組:對照組. A、B、C組大鼠連續3d鼻內滴入煙麯黴孢子懸液,四組大鼠均于第1次滴菌後第4天處死. 取內眥靜脈血鑑測大鼠免疫抑製情況,對鼻部組織行真菌培養和組織病理學觀察. 結果 免疫抑製組大鼠血中性粒細胞明顯降低,接種煙麯黴噹天中性粒細胞計數<0.1×109/L,其餘兩組變化不明顯. 病理結果顯示A組90% (9/10)大鼠鼻腔鼻竇組織被煙麯黴侵襲,10% (1/10)肺部可見侵襲;B、C、D組大鼠無鼻部煙麯黴感染,但C組中20% (2/10)大鼠肺部可見煙麯黴侵襲.真菌培養結果顯示A組71% ( 5/7)大鼠鼻部組織煙麯黴培養暘性,其他組均為陰性. 結論 單純對大鼠進行免疫抑製或單純造成大鼠鼻腔堵塞均不易造成大鼠鼻腔被真菌侵襲;免疫抑製基礎上,對大鼠鼻腔填塞Merocel海綿條後再滴入煙麯黴可以成功建立急性侵襲性真菌性鼻?鼻竇炎模型,其成模率高,模型穩定,操作簡易,有利于對此疾病在免疫、病理、藥物等方麵的深入研究.
목적 건립은정차역조작적급성침습성진균성비?비두염대서모형,이촉진대급성침습성진균성비?비두염적실험연구,지도림상. 방법 장SD대서수궤분위사조. A조,면역억제+우측비강전새Merocel해면조+우측비강적입연곡매포자현액;B조,우측비강전새Merocel해면조+우측비강적입연곡매포자현액;C조,면역억제+우측비강적입연곡매포자현액;D조:대조조. A、B、C조대서련속3d비내적입연곡매포자현액,사조대서균우제1차적균후제4천처사. 취내자정맥혈감측대서면역억제정황,대비부조직행진균배양화조직병이학관찰. 결과 면역억제조대서혈중성립세포명현강저,접충연곡매당천중성립세포계수<0.1×109/L,기여량조변화불명현. 병리결과현시A조90% (9/10)대서비강비두조직피연곡매침습,10% (1/10)폐부가견침습;B、C、D조대서무비부연곡매감염,단C조중20% (2/10)대서폐부가견연곡매침습.진균배양결과현시A조71% ( 5/7)대서비부조직연곡매배양양성,기타조균위음성. 결론 단순대대서진행면역억제혹단순조성대서비강도새균불역조성대서비강피진균침습;면역억제기출상,대대서비강전새Merocel해면조후재적입연곡매가이성공건립급성침습성진균성비?비두염모형,기성모솔고,모형은정,조작간역,유리우대차질병재면역、병리、약물등방면적심입연구.
Objective To develop a rat model of acute invasive fungal rhinosinusitis ( AIFR) which is stable and easy to operate in order to promote the basic and clinical researches of AIFR.Methods Sprague?Dawley (SD) rats were divided randomly into four groups.Immunosuppression,right nasal packing Merocel sponge and nasal inoculation with Aspergillus fumigatus spores were all in?volved in group A.Only right nasal packing Merocel sponge and nasal inoculation with A.fumigatus spores were involved in group B. Only immunosuppression and nasal inoculation with A.fumigatus spores were involved in group C.No treatment was involved in group D.A.fumigatus was dropped into rat nasal for three consecutive days in group A,B and C.All rats were killed four days after the first fungi intranasal instillation. Hematology, fungal culture and histopathology investigations were performed. Results The neutrophil quantities reduced after immunosupression,and less than 0.1×109/L on the first administration day of A.fumigatus spores.An AIFR rat model was established successfully only in group A with an incidence rate of 90% (9/10).A.fumigatus invasion was also observed in 10% (1/10) of the lungs in group A and 20% (2/10) in group C.Positive rates of fungal culture of nasal tissue was about 71%(5/7) in group A,while the remaining groups was zero.Conclusion Immunosupression,nasal obstruction,and fungal inoculation were the three essential conditions for successfully developping a stable AIFR rat model.This model is stable,easy to operate,and closely mimics the pathophysiology of anthropic AIFR.It can be used in a further study in immunity,pathology,drug of AIFR.
