中国真菌学杂志
中國真菌學雜誌
중국진균학잡지
Chinese Journal of Mycology
2015年
5期
279-282
,共4页
贺羽%黄梦雅%胡青碧%周汛
賀羽%黃夢雅%鬍青碧%週汛
하우%황몽아%호청벽%주신
孢子丝菌%形态学%系统发育%球形孢子丝菌
孢子絲菌%形態學%繫統髮育%毬形孢子絲菌
포자사균%형태학%계통발육%구형포자사균
Sporothrix%morphology%phylogeny%Sporothrix globosa
目的 对分离自我国南、北方地区50株临床孢子丝菌进行菌种鉴定. 方法 分离菌株分别进行25℃恒温培养和玻片小培养,肉眼和镜下观察形态特征;同时提取菌丝相基因组DNA,用PCR分别扩增部分微管蛋白 (β?tubulin)基因和核糖体内部转录间隔区 ( Ribosomal Internal Transcribed Spacer,ITS) ,扩增产物进行测序,并采用最大似然法 ( maximum likeli?hood,ML)和邻接法 ( neighbor?joining,NJ)构建联合β?tubulin基因和ITS区域的系统发育树. 结果 结合形态学及系统发育分析,50株菌均鉴定为球形孢子丝菌 (Sporothrix globosa,S.globosa). 结论 球形孢子丝菌是目前我国南、北方地区孢子丝菌病的主要致病菌种,为进一步明确我国孢子丝菌病致病菌种的分布提供了依据.
目的 對分離自我國南、北方地區50株臨床孢子絲菌進行菌種鑒定. 方法 分離菌株分彆進行25℃恆溫培養和玻片小培養,肉眼和鏡下觀察形態特徵;同時提取菌絲相基因組DNA,用PCR分彆擴增部分微管蛋白 (β?tubulin)基因和覈糖體內部轉錄間隔區 ( Ribosomal Internal Transcribed Spacer,ITS) ,擴增產物進行測序,併採用最大似然法 ( maximum likeli?hood,ML)和鄰接法 ( neighbor?joining,NJ)構建聯閤β?tubulin基因和ITS區域的繫統髮育樹. 結果 結閤形態學及繫統髮育分析,50株菌均鑒定為毬形孢子絲菌 (Sporothrix globosa,S.globosa). 結論 毬形孢子絲菌是目前我國南、北方地區孢子絲菌病的主要緻病菌種,為進一步明確我國孢子絲菌病緻病菌種的分佈提供瞭依據.
목적 대분리자아국남、북방지구50주림상포자사균진행균충감정. 방법 분리균주분별진행25℃항온배양화파편소배양,육안화경하관찰형태특정;동시제취균사상기인조DNA,용PCR분별확증부분미관단백 (β?tubulin)기인화핵당체내부전록간격구 ( Ribosomal Internal Transcribed Spacer,ITS) ,확증산물진행측서,병채용최대사연법 ( maximum likeli?hood,ML)화린접법 ( neighbor?joining,NJ)구건연합β?tubulin기인화ITS구역적계통발육수. 결과 결합형태학급계통발육분석,50주균균감정위구형포자사균 (Sporothrix globosa,S.globosa). 결론 구형포자사균시목전아국남、북방지구포자사균병적주요치병균충,위진일보명학아국포자사균병치병균충적분포제공료의거.
Objective To identify 50 Sporothrix clinical isolates in Northern and Southern of China.Methods All isolates from patients were subcultured at 25℃ and morphological characteristic was analysed by microscope and visual inspection. The total ge?nomic DNA was extracted to amplify the partialβ?tubulin gene and the internal transcribed ( ITS) region by polymerase chain reac?tion (PCR).The phylogenetic tree was constructed by DNA sequences analyses based on datasets of ITS and a combined ITS and partial β?tubulin region using maxium likehood (ML) and neighbor?joining (NJ) methods with MEGA5 software.The Reference se?quences used for phylogenetic analyses were retrieved from GenBank. Result The macroscopic morphologies and microscopic fea?tures of all isolates were comformed to Sporothrix spp.The phylogenetic tree showed that all of the isolates were clustered in a distinct clade with a type of Sporothrix globosa.Conclusion S.globosa may be the major Sporothrix existing in northern and southern of Chi?na,which can provide basis for the epidemiological investigation of Sporothrix.
