CAJ | 학술논문

为探讨刚毛柽柳真核翻译起始因子1A （TheIF1A）基因对植物逆境胁迫响应的调控作用，对TheIF1A 基因启动子盐胁迫下的应答表达情况进行分析，比较转 TheIF1A 基因启动子拟南芥在不同浓度盐胁迫及不同时间后 GUS 染色和酶活差异。结果显示：TheIF1A 启动子在短时间内即对400 mmol ／L NaCl 胁迫做出应答，主要表现为表达受抑制，各组织中老叶的 GUS 酶活性最高；100 mmol ／L NaCl 胁迫下，TheIF1A 启动子活性也被明显抑制，胁迫20 h 后，GUS 酶活性恢复到较高水平，各组织中，根中的活性最低。研究表明，TheIF1A 启动子的表达能对盐胁迫作出应答，但在低盐和高盐胁迫下的应答方式存在一定的差异性。
위탐토강모정류진핵번역기시인자1A （TheIF1A）기인대식물역경협박향응적조공작용，대TheIF1A 기인계동자염협박하적응답표체정황진행분석，비교전 TheIF1A 기인계동자의남개재불동농도염협박급불동시간후 GUS 염색화매활차이。결과현시：TheIF1A 계동자재단시간내즉대400 mmol ／L NaCl 협박주출응답，주요표현위표체수억제，각조직중로협적 GUS 매활성최고；100 mmol ／L NaCl 협박하，TheIF1A 계동자활성야피명현억제，협박20 h 후，GUS 매활성회복도교고수평，각조직중，근중적활성최저。연구표명，TheIF1A 계동자적표체능대염협박작출응답，단재저염화고염협박하적응답방식존재일정적차이성。
Eukaryotic translation initiation factor 1A in Tamarix hispida (TheIF1A)invovled in the regulation to abiotic stress.In this study,the TheIF1A promoter expression patterns were analyzed under salt stress to explore the genes functions under stress and then,GUS activities among different concentration NaCl and various stress times in the transgenic TheIF1A promoter T4 Arabidopsis were compared.The results showed that TheIF1A promot-er could quickly response to NaCl with 400 mmol /L.The GUS activity was decreased after 15 min treated by NaCl stress.And the highest GUS activities were showed in leaves.The expression of TheIF1A promoter was also obvi-ously decreased under 100 mmol /L NaCl stress.Then the GUS activities were increased.After treated by 20 h,it was obvious higher than 0 h treatment.Among the 3 organs (roots,leaves and petioles),the GUS activity in root was the lowest.These results indicated that the expressions of TheIF1A promoter could response to salt stress,while the expression patters were difference between high and low concentration NaCl stresses.