CAJ | 학술논문

目的：比较不同程度干眼患者共焦显微镜下角膜内树突状细胞的密度、活化细胞比例,以期进一步揭示干眼的炎症机制,为干眼的临床诊断和治疗提供依据。方法：选择临床诊断为轻度、中度及重度干眼患者各20例,对其双眼进行共焦显微镜检查,对每幅共焦图片内角膜上皮下的树突状细胞进行计数,并换算成细胞密度(个/ mm2)。比较不同程度干眼患者的树突状细胞密度的平均值。同时对不同程度干眼共焦显微镜下活化的树突状细胞比例进行统计比较。结果：轻度干眼上皮下树突状细胞密度为26.7±8.8个/mm2,其中活化细胞比率29.0±3.7%；中度干眼树突状细胞密度为69.6±11.1个/mm2,其中活化细胞比率83.5±7.6%；重度干眼树突状细胞密度为212.2±31.0个/mm2,其中活化细胞比率97.8±1.9%。干眼患者随其严重程度增加,角膜上皮下树突状细胞密度亦呈增加趋势,并且其上皮下活化的树突状细胞比例亦增加,具有统计学差异。结论：共焦显微镜下角膜内树突状细胞是干眼炎症的重要标志之一,其密度的变化和活化细胞的比例可作为评估干眼炎症程度的有效指标。
목적：비교불동정도간안환자공초현미경하각막내수돌상세포적밀도、활화세포비례,이기진일보게시간안적염증궤제,위간안적림상진단화치료제공의거。방법：선택림상진단위경도、중도급중도간안환자각20례,대기쌍안진행공초현미경검사,대매폭공초도편내각막상피하적수돌상세포진행계수,병환산성세포밀도(개/ mm2)。비교불동정도간안환자적수돌상세포밀도적평균치。동시대불동정도간안공초현미경하활화적수돌상세포비례진행통계비교。결과：경도간안상피하수돌상세포밀도위26.7±8.8개/mm2,기중활화세포비솔29.0±3.7%；중도간안수돌상세포밀도위69.6±11.1개/mm2,기중활화세포비솔83.5±7.6%；중도간안수돌상세포밀도위212.2±31.0개/mm2,기중활화세포비솔97.8±1.9%。간안환자수기엄중정도증가,각막상피하수돌상세포밀도역정증가추세,병차기상피하활화적수돌상세포비례역증가,구유통계학차이。결론：공초현미경하각막내수돌상세포시간안염증적중요표지지일,기밀도적변화화활화세포적비례가작위평고간안염증정도적유효지표。
Objective:To objective the density of dendritic cellsand the proportion of activated cells of dif-ferent degree of dry eye patients in vivo confocal microscopy,so as to investigate the mechanism of dry eye ,to pro-vide the basis for clinical diagnosis and treatment of dry eye.Methods:A case series with 60 consecutive cases of dif-ferent degrees dry eye patients was investigated,examined with confocal microscopy in vivo,and at the same time the density of dendritic cells and the proportion of activated cells was recorded and analyzed with SPSS1 7.0 .Results:In mild dry eye,dendritic cell density was 26.7 ± 8.8/ mm2 ,with activated cell 29.0 ± 3.7%;In moderate dry eye dendritic cell density was 69.6 ± 1 1.1 / mm2 ,with activate cell 83.5±7.6%;In severe dry eye dendritic cell den-sity was 212.2 ± 31.0 / mm2 ,with activate cell 97.8±1.9%.The data showed that the corneal dendritic cell den-sityand the proportion of activated cells were found increased in more severe dry eye,with statistically significant difference .Conclusion:In vivo confocal microscope,dendritic cells is an important sign of dry eye inflammation.The proportion of activated cells and its density can be used as effective indicators to assess the degree of dry eye.