CAJ | 학술논문

目的：评价 PCR-RDB 技术在丙型肝炎病毒(HCV)基因型检测中的临床实际应用价值。方法：随机收集经实时荧光定量聚合酶链式反应(PCR)检测 HCV RNA 含量大于1×103 IU/ml 血清样本总计158份,其中无临床症状丙型肝炎病毒携带者90例,慢性丙型肝炎(CHC)患者48例,肝硬化(HS)患者16例,肝癌(LC)患者4例。全部血清样本应用 PCR 和反向斑点杂交技术(RDB)进行 HCV 基因分型检测,并对检测结果进行统计学分析。结果：所有标本 HCV 基因分型成功,其中1b 型78例,2a 型65份,3a 型3例,3b 型2例,6a 型2例,1b 和2a 混合感染型8例。不同疾病组 HCV 不同基因型分布差异无统计学意义。结论：HCV 基因型与疾病类型无相关性；PCR-RDB 杂交技术适用于临床实验室开展 HCV 基因型检测。
목적：평개 PCR-RDB 기술재병형간염병독(HCV)기인형검측중적림상실제응용개치。방법：수궤수집경실시형광정량취합매련식반응(PCR)검측 HCV RNA 함량대우1×103 IU/ml 혈청양본총계158빈,기중무림상증상병형간염병독휴대자90례,만성병형간염(CHC)환자48례,간경화(HS)환자16례,간암(LC)환자4례。전부혈청양본응용 PCR 화반향반점잡교기술(RDB)진행 HCV 기인분형검측,병대검측결과진행통계학분석。결과：소유표본 HCV 기인분형성공,기중1b 형78례,2a 형65빈,3a 형3례,3b 형2례,6a 형2례,1b 화2a 혼합감염형8례。불동질병조 HCV 불동기인형분포차이무통계학의의。결론：HCV 기인형여질병류형무상관성；PCR-RDB 잡교기술괄용우림상실험실개전 HCV 기인형검측。
Objective:To evaluate the clinical practical application value of polymerase chain reaction and reverse dot blot(PCR-RDB)method on genotyping HCV in different disease groups.Methods:We conducted a co-hort study with 1 58 participants including 90 HCV carriers without clinical symptom,48 patients with chronic hepa-titis C,1 6 patients with hepatic sclerosis and 5 patients with liver cancer.The HCV RNA levels of all sera samples were confirmed higher than 1 × 10 3 IU/ml by real-time fluorescent quantitative polymerase chain reaction(PCR). HCV genotyping was carried out using type-specific primers by means of polymerase chain reaction and reverse dot blot(RDB).And the results were statistically analyzed with GraphPad Prism 5.0 software.Results:All 1 58 speci-mens were genotyped successfully.Among them,78 cases were genotype 1b,65 cases were genotype 2a,3 cases were genotype 3a,2 cases were genotype 3b,2 cases were genotype 6a,8 cases were mixed genotypes 1b and 2a. There was no significant difference among the different disease groups about the distribution of HCV genotypes. Conclusion:There is no relationship between the HCV genotypes and disease types.PCR-RDB was a suitable meth-od for the HCV genotyping in clinical laboratories.