CAJ | 학술논문

目的：探讨自身特异性抗体筛查试验间接免疫荧光法(IIF)与确认试验线性免疫印迹法(LIA)检测结果的相关性。方法：用 IIF 法作为抗核抗体(ANA)的筛查试验,用 LIA 法作为抗核抗体谱(ANAs)特异性抗体的确认试验,对自身免疫性疾病组(AID)及非 AID 疾病对照组进行检测分析,并将检测结果分为四组：IIF-ANA+/LIA+组,IIF-ANA+/LIA-组,IIF-ANA-/LIA+组和 IIF-ANA-/LIA-组。结果：①在611例 AID 组中,IIF-ANA+/LIA+336例(55%),IIF-ANA+/LIA-116例(19%),IF-ANA-/LIA+55例(9%),IIF-ANA-/LIA-104例(17%)。②在100例非 AID 组中,IIF-ANA+/LIA+2例(2%),IIF-ANA+/LIA-7例(7%),IIF-ANA-/LIA+3例(3%),IIF-ANA-/LIA-88例(88%),总体符合率90%。AID 组与非 AID 组自身抗体的检测结果比较差异有统计学意义。③ IIF-ANA-/LIA+组中,以抗 SSA 抗体、抗 Ro-52抗体、抗 ds-DNA 抗体、抗组蛋白抗体、抗 Jo-1抗体为主要检测出的阳性自身抗体。AID 组 IIF-ANA+/LIA-中,ANA检测核型以均质型、斑点型为主,混合核型以均质斑点型为主,荧光检测滴度以1∶40~1∶320为主；非 AID 组 IIF-ANA+/LIA-中,核型以斑点型为主,荧光滴度以1∶40~1∶320为主。结论：AID 患者可能会出现 IIF-ANA 检测阳性而 LIA-ANAs 检测阴性或者 IIF-ANA 检测阴性而 LIA-ANAs 检测阳性,检查除了进行 IIF 筛查 ANA 外,还需进行自身抗体谱的特异性抗体确认实验。
목적：탐토자신특이성항체사사시험간접면역형광법(IIF)여학인시험선성면역인적법(LIA)검측결과적상관성。방법：용 IIF 법작위항핵항체(ANA)적사사시험,용 LIA 법작위항핵항체보(ANAs)특이성항체적학인시험,대자신면역성질병조(AID)급비 AID 질병대조조진행검측분석,병장검측결과분위사조：IIF-ANA+/LIA+조,IIF-ANA+/LIA-조,IIF-ANA-/LIA+조화 IIF-ANA-/LIA-조。결과：①재611례 AID 조중,IIF-ANA+/LIA+336례(55%),IIF-ANA+/LIA-116례(19%),IF-ANA-/LIA+55례(9%),IIF-ANA-/LIA-104례(17%)。②재100례비 AID 조중,IIF-ANA+/LIA+2례(2%),IIF-ANA+/LIA-7례(7%),IIF-ANA-/LIA+3례(3%),IIF-ANA-/LIA-88례(88%),총체부합솔90%。AID 조여비 AID 조자신항체적검측결과비교차이유통계학의의。③ IIF-ANA-/LIA+조중,이항 SSA 항체、항 Ro-52항체、항 ds-DNA 항체、항조단백항체、항 Jo-1항체위주요검측출적양성자신항체。AID 조 IIF-ANA+/LIA-중,ANA검측핵형이균질형、반점형위주,혼합핵형이균질반점형위주,형광검측적도이1∶40~1∶320위주；비 AID 조 IIF-ANA+/LIA-중,핵형이반점형위주,형광적도이1∶40~1∶320위주。결론：AID 환자가능회출현 IIF-ANA 검측양성이 LIA-ANAs 검측음성혹자 IIF-ANA 검측음성이 LIA-ANAs 검측양성,검사제료진행 IIF 사사 ANA 외,환수진행자신항체보적특이성항체학인실험。
Objective:To explore the correlation of the specific antibody results between screening test of IIF and validation test of LIA.Methods:IIF method as ANA screening test,using LIA method as the validation test of ANAs ,Analysis the group of AID and the disease control group of not AID,the test results can be divided into four groups:group IIF ANA + / LIA +,group IIF ANA + / LIA-,group IIF-ANA-/ LIA +,group IIF-ANA-/ LIA-.Results:①In 61 1 cases of AID groups:IIF ANA + / LIA + had 336 patients (55%),IIF ANA+ / LIA-had 1 1 6 patients (1 9%),IIF ANA-/ LIA + had 55 cases (9%),IIF ANA-/ LIA-had 104 patients (1 7%).②In 100 cases of the not AID group:IIF ANA + /LIA + had 2 cases (2%),IIF ANA + /LIA-had 7 cases (7%),IIF ANA-/ LIA + had 3 cases (3%),IIF ANA-/LIA-have 88 patients (88%),the overall coinci-dence rate was 90%.Two groups autoantibodies detection results had significant statistical significance difference .③In the IIF ANA-/LIA + group,there are anti SSA antibody,anti Ro-52 antibody,anti ds-DNA antibody,anti-histonesantibody,anti Jo-1 antibody as main detected positive autoantibodies.In the AID groups of IIF ANA + /LIA-,ANA detection karyotype was given priority to with homogeneous,spots,and mixed karyotype was given priority to with homogeneous spots type,and the fluorescence detection of antibody titer was given priority to with 1∶40~1∶320;In the not AID group IIF ANA +/LIA-,karyotype was given priority to with spot type,the fluo-rescence detection of antibody titer was given priority to with 1∶40~1∶320.Conclusion:In the clinical test of AID may appear the IIF test positive for the ANA and LIA-ANAs test negative or IIF-ANA test negative and LIA-AN-As test positive.So in the clinical test of AID,in addition to the IIF screening test,you also need to do the confirma-tion experiment of autoantibodies spectrum simultaneously,so as to avoid a misdiagnosis because ofthe single test.