CAJ | 학술논문

目的了解2014年广东省食源性副溶血弧菌分离株的血清型别、抗生素敏感性、毒力基因携带情况以及分子分型特征.方法对60株副溶血弧菌分离株进行血清分型、抗生素敏感性试验及检测耐热直接溶血素基因(tdh)和耐热相关溶血素基因(trh),并进行脉冲场凝胶电泳(PFGE)和多位点序列(MLST)分型.结果 60株分离株可分为13个血清型,主要型别为O3:K6、O4:K8、O1:K36和O4:KUT;对氨苄西林、磺胺复合物和头孢噻吩的耐药率分别为100.0％、43.3％和28.3％,有56.7％(34/60)的菌株对2类及以上抗生素同时耐药,2株菌对3类抗生素同时耐受.毒力基因PCR检测发现,有63.3％(38/60)的分离株为tdh+ trh-菌株,仅1株为tdh+ trh+菌株.分子分型显示,经NotⅠ酶消化后,60株副溶血弧菌可产生48个PFGE谱型,可分为3个聚类(ClusterA、B和C).其中ClusterB的菌株主要分离自食源性疾病监测中心散发病例,血清型以O3:K6为主,PFGE谱型的相似度在62.6％～ 100.0％;ClusterC主要是由4株O4: K8型菌株组成,谱型相似度为56.7％～ 62.5％.60株菌MLST分型可分为26个ST型,其中33株菌为ST-3型,主要是O3:K6和O1:K36菌株;4株O4: K8菌株聚集成另一个不同于ST-3型的相对优势的菌群.结论 2014年广东省副溶血弧菌菌型多样性可能是疾病高发的原因之一,O4: K8型菌株的分子特征与其他优势血清型别明显不同,应高度警惕该型菌株引起暴发的可能.
목적 료해2014년광동성식원성부용혈호균분리주적혈청형별、항생소민감성、독력기인휴대정황이급분자분형특정.방법 대60주부용혈호균분리주진행혈청분형、항생소민감성시험급검측내열직접용혈소기인(tdh)화내열상관용혈소기인(trh),병진행맥충장응효전영(PFGE)화다위점서렬(MLST)분형.결과 60주분리주가분위13개혈청형,주요형별위O3:K6、O4:K8、O1:K36화O4:KUT;대안변서림、광알복합물화두포새분적내약솔분별위100.0％、43.3％화28.3％,유56.7％(34/60)적균주대2류급이상항생소동시내약,2주균대3류항생소동시내수.독력기인PCR검측발현,유63.3％(38/60)적분리주위tdh+ trh-균주,부1주위tdh+ trh+균주.분자분형현시,경NotⅠ매소화후,60주부용혈호균가산생48개PFGE보형,가분위3개취류(ClusterA、B화C).기중ClusterB적균주주요분리자식원성질병감측중심산발병례,혈청형이O3:K6위주,PFGE보형적상사도재62.6％～ 100.0％;ClusterC주요시유4주O4: K8형균주조성,보형상사도위56.7％～ 62.5％.60주균MLST분형가분위26개ST형,기중33주균위ST-3형,주요시O3:K6화O1:K36균주;4주O4: K8균주취집성령일개불동우ST-3형적상대우세적균군.결론 2014년광동성부용혈호균균형다양성가능시질병고발적원인지일,O4: K8형균주적분자특정여기타우세혈청형별명현불동,응고도경척해형균주인기폭발적가능.
Objective To investigate the serotypes, antimicrobial resistance, virulence genes and molecular characteristics of food-borne Vibrio parahaemolytieus isolated in Guangdong province in 2014.Methods Sixty V.parahaemolyticus strains were tested in this study.The serotyping and antibiotic resistance test were conducted, while the tdh and trh gens of the strains were detected with conventional PCR, and pulsed field gel electrophoresis (PFGE) and multiple locus sequence typing (MLST) were conducted too.Results The 60 strains belonged to 13 serotypes, the major serotypes included O3 : K6, O4: K8, O 1 : K36 and O4: KUT.The antibiotic resistance test indicated the isolates were highly resistant to ampicillin (100.0％), sulfonamides (43.3％) and cefalotin (28.3％).Up to 56.7％(34/60) of the strains were resistant to two or more antibiotics, and 2 strains showed resistance to three antibiotics.The virulence gene detection indicated that 63.3％ (38/60) of the strains carded tdh+trh-, while only 1 strain carried tdh+trh+.The 60 strains digested by Not I belonged to 48 different PFGE patterns and 3 clusters.The cluster B included the strains isolated from sporadic food borne cases with serotype of O3:K6 and similarity of 62.6％-100.0％.The cluster C included O4:K8 strains with the PFGE pattem similarity of 56.7％-62.5％.The MLST indicated that the 60 strains had 26 sequence types (STs).The ST-3 was predominant, including 33 strains (serotypes O3:K6 and O1 : K36).The four O4:K8 strains formed another predominant colony, which was different from ST-3.Conclusion The etiologic characteristics of V.parahaemolyticus varied, which might be one of the reasons for high incidence of food-bome V.parahaemolyticus infection in Guangdong.The molecular characteristics of O4: K8 strain were differem from the other predominant serotypes.Close attention should be paid to the possible outbreak caused by O4: K8 strain in this area.