CAJ | 학술논문

目的：研究粘质沙雷菌的耐药性与细胞的主动外排机制及细菌膜蛋白缺失相关性。方法：收集对亚胺培南和美罗培南耐药的粘质沙雷菌株15株,应用 PCR 扩增和 DNA 序列分析,采用 E 试验观察氰氯苯腙对亚胺培南最低抑菌浓度(MIC)的影响,以研究细胞内是否存在主动外排机制和膜蛋白缺失现象。结果：当 CCCP 存在时,其中7株粘质沙雷菌亚胺培南,美罗培南的MIC 值下降,提示存在主动外排机制。其中有1株 OprD2蛋白缺失,1株外排泵 MexB 基因阳性。结论：耐碳青霉烯类药物的粘质沙雷菌的耐药性与细胞的主动外排机制和膜蛋白缺失有关。
목적：연구점질사뢰균적내약성여세포적주동외배궤제급세균막단백결실상관성。방법：수집대아알배남화미라배남내약적점질사뢰균주15주,응용 PCR 확증화 DNA 서렬분석,채용 E 시험관찰청록분종대아알배남최저억균농도(MIC)적영향,이연구세포내시부존재주동외배궤제화막단백결실현상。결과：당 CCCP 존재시,기중7주점질사뢰균아알배남,미라배남적MIC 치하강,제시존재주동외배궤제。기중유1주 OprD2단백결실,1주외배빙 MexB 기인양성。결론：내탄청매희류약물적점질사뢰균적내약성여세포적주동외배궤제화막단백결실유관。
Objective:Study the drug resistance of Serratia marcescens whether related to the cells active efflux mechanism and the absence of bacterial membrane proteins or not.Methods:collected 1 5 strains Serratia marcescens that resist Imipenem and Meropenem ,application PCR amplification and DNA sequence analysis.adopt E test experiment observe the influence of CCCP for Imipenem minimal inhibitory concentration.in order to study intracellular was exist active efflux mechanism and the absence of membrane proteins or not.Results:When CCCP exist,there were 7 strains serratia marcescens for imipenem and Meropenem’MIC value decreased,prompting exist active efflux mechanism bacterial,and 1 strain absent OprD2 protein,1 strain efflux pump MexB gene positive.Con-clusion:The resistance of Serratia marcescens that resist Carbapenemase related to the cells active efflux mechanism and the absence of bacterial membrane proteins .