临床医学
臨床醫學
림상의학
Clinical Medicine
2015年
11期
1-4
,共4页
高洁%王涛%肖海%娄桂予%郭梁洁%吴东%李涛%秦利涛%廖世秀
高潔%王濤%肖海%婁桂予%郭樑潔%吳東%李濤%秦利濤%廖世秀
고길%왕도%초해%루계여%곽량길%오동%리도%진리도%료세수
亚甲基四氢叶酸还原酶%甲硫氨酸合成还原酶%复发性流产%基因多态性
亞甲基四氫葉痠還原酶%甲硫氨痠閤成還原酶%複髮性流產%基因多態性
아갑기사경협산환원매%갑류안산합성환원매%복발성유산%기인다태성
Methylenetetrahydrofolate reductase%Methionine synthase reductase%Recurrent spontaneous abrotion%Gene polymorphism
目的：研究叶酸代谢基因多态性在河南地区育龄妇女的分布情况，探索其与不明原因复发性流产的相关性。方法选取无合并症的423名孕妇（对照组）和不明原因复发性流产就诊的378名妇女（观察组），采用 PCR 荧光探针法检测亚甲基四氢叶酸还原酶基因（MTHFR）C677T、A1298C 位点及甲硫氨酸合成还原酶基因（MTRR）A566G 位点的多态性分布。结果观察组 MTHFR 677位点携带突变 T 基因、MTHFR 1298位点携带突变 C 基因与对照组比较差异有统计学意义，观察组 MTR 566位点携带 G 基因与对照组比较差异未见统计学意义。当有两个基因位点同时发生突变时，增加了复发性流产的概率。结论 MTHFR C677T 和 A1298C 与育龄妇女发生不明原因复发性流产有一定的关联。
目的：研究葉痠代謝基因多態性在河南地區育齡婦女的分佈情況，探索其與不明原因複髮性流產的相關性。方法選取無閤併癥的423名孕婦（對照組）和不明原因複髮性流產就診的378名婦女（觀察組），採用 PCR 熒光探針法檢測亞甲基四氫葉痠還原酶基因（MTHFR）C677T、A1298C 位點及甲硫氨痠閤成還原酶基因（MTRR）A566G 位點的多態性分佈。結果觀察組 MTHFR 677位點攜帶突變 T 基因、MTHFR 1298位點攜帶突變 C 基因與對照組比較差異有統計學意義，觀察組 MTR 566位點攜帶 G 基因與對照組比較差異未見統計學意義。噹有兩箇基因位點同時髮生突變時，增加瞭複髮性流產的概率。結論 MTHFR C677T 和 A1298C 與育齡婦女髮生不明原因複髮性流產有一定的關聯。
목적：연구협산대사기인다태성재하남지구육령부녀적분포정황，탐색기여불명원인복발성유산적상관성。방법선취무합병증적423명잉부（대조조）화불명원인복발성유산취진적378명부녀（관찰조），채용 PCR 형광탐침법검측아갑기사경협산환원매기인（MTHFR）C677T、A1298C 위점급갑류안산합성환원매기인（MTRR）A566G 위점적다태성분포。결과관찰조 MTHFR 677위점휴대돌변 T 기인、MTHFR 1298위점휴대돌변 C 기인여대조조비교차이유통계학의의，관찰조 MTR 566위점휴대 G 기인여대조조비교차이미견통계학의의。당유량개기인위점동시발생돌변시，증가료복발성유산적개솔。결론 MTHFR C677T 화 A1298C 여육령부녀발생불명원인복발성유산유일정적관련。
Objective To analyze the relationships between gene polymorphism involved in folate metbolism and the maternal risks for unexplained recurrent spontaneous abortion in Henan province. Methods A case control study was conducted. The pol-ymorphism of C677T,A1298C in methyleneterahydrofolate reductase( MTHFR)and A66G in methionine synthase reductase (MTRR)were analyzed among 378 females of recurrent spontaneous abortion(observation group)and 423 normal females(con-trol group)by polymerase chain reaction(PCR)fluorescence probe. Results The distribution frequency of MTHFR C677T and A1298C genotype in the observation group were significantly increased compared with the control group. The distribution frequen-cy of MTRR A66G genotype had no statistical difference between the two groups. The interactive analysis of 2 genes indicated that simultaneous carrying the MTHFR 677,1298 mutation site and MTRR A66G mutation site increased the possibility of recur-rent spontaneous abortions occurrence. Conclusion MTHFR C677T and A1298 have a certain correlation with female unex-plained recurrent spontaneous abortion.
