CAJ | 학술논문

目的明确MicroRNA218-Robo1通路在动物体内对乳腺癌的抑制功能及其机制.方法 BALB/c-nu/nu雌性裸小鼠40只,分为4组,每组动物分别用转染后高表达MicroRNA218的MDA-MB-231细胞、转染后共同高表达MicroRNA218和Robo1基因的MDA-MB-231细胞、转染后敲除Robo1基因的MDA-MB-231细胞以及阴性对照的MDA-MB-231细胞.从接种当天开始,每2周采用精诺真活体成像系统(IVIS 200)观测皮下肿瘤细胞的生长情况.TUNEL染色检测凋亡细胞,CD31染色检测肿瘤微血管密度,Ki-67染色检测肿瘤细胞增殖情况.结果 MicroRNA218组的肿瘤体积明显小于对照组,Robo1敲除组的肿瘤体积明显小于共同高表达MicroRNA218和Robo1组,差异有统计学意义;MicroRNA218和Robo1敲除组较对照组,乳腺癌细胞凋亡增多,细胞增殖和新生血管产生受到抑制.结论 MicroRNA218能够通过调节Robo1的表达抑制乳腺癌肿瘤细胞的迁移和侵袭.
목적 명학MicroRNA218-Robo1통로재동물체내대유선암적억제공능급기궤제.방법 BALB/c-nu/nu자성라소서40지,분위4조,매조동물분별용전염후고표체MicroRNA218적MDA-MB-231세포、전염후공동고표체MicroRNA218화Robo1기인적MDA-MB-231세포、전염후고제Robo1기인적MDA-MB-231세포이급음성대조적MDA-MB-231세포.종접충당천개시,매2주채용정낙진활체성상계통(IVIS 200)관측피하종류세포적생장정황.TUNEL염색검측조망세포,CD31염색검측종류미혈관밀도,Ki-67염색검측종류세포증식정황.결과 MicroRNA218조적종류체적명현소우대조조,Robo1고제조적종류체적명현소우공동고표체MicroRNA218화Robo1조,차이유통계학의의;MicroRNA218화Robo1고제조교대조조,유선암세포조망증다,세포증식화신생혈관산생수도억제.결론 MicroRNA218능구통과조절Robo1적표체억제유선암종류세포적천이화침습.
Objective To observe the migration and inhibition mechanism of MicroRNA218-Robo1 pathway for breast cancer.Methods A total of 40 BALB/c-nu/nu female mice were randomly divided into four groups.Each group was transfected over-expression MicroRNA218 MDA-MB-231 breast cancer cells, co-over-expression MicroRNA218 and Robo1 MDA-MB-231 breast cancer cells, knock-down Robo1 MDA-MB-231 breast cancer cells and the control MDA-MB-231 breast cancer cells.The tumor volume was examined every two weeks.Results Tumor volume of MicroRNA218 group was obviously less than control group, tumor volume of Robo1 knock out group was obviously less than common MicroRNA218 high expression and Robo1 group, the difference was statistically significant;MicroRNA218 and Robol knockout group than the control group, the increase in breast cancer cells apoptosis, cell proliferation and angiogenesis is restrained.Conclusions MicroRNA218 inhibited the migration of breast cancer by down-regulating the expression of Robo1.