CAJ | 학술논문

目的探讨脂联素是否通过AMPK通路介导线粒体自噬调控炎症反应. 方法小鼠腹腔巨噬细胞细胞分为五组:空白对照组(A组)、脂多糖组(B组)、脂联素预处理组(C组)、脂联素预处理+AMPK拮抗剂组( D组)及AMPK拮抗剂组( E组). 细胞接种于60 mm培养皿中,分别予以培基,LPS,APN联合LPS, LPS、APN联合AMPK拮抗剂Compound C,LPS联合Compound C孵育,Western blot法测定AMPK及LC3B蛋白含量,酶联免疫吸附试验(ELISA)检测 IL-1β及IL-6水平. 结果与A组比较,B组AMPK及LC3B蛋白含量、IL-1β及IL-6水平增加( P<0.05);与B组比较,C组AMPK及LC3B蛋白含量升高(P<0.05),IL-1β及IL-6 水平降低(P<0.05);与C组比较,D组AMPK及LC3B蛋白含量降低,IL-1β及IL-6 水平升高(P<0.05 ). 结论脂联素可调控LPS介导的炎症反应,其机制可能与激活AMPK通路、增强线粒体自噬有关.
목적 탐토지련소시부통과AMPK통로개도선립체자서조공염증반응. 방법 소서복강거서세포세포분위오조:공백대조조(A조)、지다당조(B조)、지련소예처리조(C조)、지련소예처리+AMPK길항제조( D조)급AMPK길항제조( E조). 세포접충우60 mm배양명중,분별여이배기,LPS,APN연합LPS, LPS、APN연합AMPK길항제Compound C,LPS연합Compound C부육,Western blot법측정AMPK급LC3B단백함량,매련면역흡부시험(ELISA)검측 IL-1β급IL-6수평. 결과 여A조비교,B조AMPK급LC3B단백함량、IL-1β급IL-6수평증가( P<0.05);여B조비교,C조AMPK급LC3B단백함량승고(P<0.05),IL-1β급IL-6 수평강저(P<0.05);여C조비교,D조AMPK급LC3B단백함량강저,IL-1β급IL-6 수평승고(P<0.05 ). 결론 지련소가조공LPS개도적염증반응,기궤제가능여격활AMPK통로、증강선립체자서유관.
Objective To investigate whether adiponectin would mediate inflammation by regulating mitochondrial autophagy through AMPK pathway .Methods Mouse peritoneal macrophages were divided into five groups: control group ( C group ) , model group ( LPS group ) , Adiponectin pretreatment group (APN group), Adiponectin-pretreatment+AMPK antagonist group (D group) and AMPK antagonist group (Compound C group).Peritoneal macrophages were cultured in 60 mm dishes, treated with LPS, APN preconditioning followed by LPS , AMPK antagonist Compound C preconditioning followed by APN and LPS , and Compound C preconditioning followed by LPS .Western blot method was used to determine the content of AMPK and LC3B, enzyme-linked immune adsorption assay (ELISA) was used to detect the level of IL -1 beta and IL -6.Results Compared with C group , AMPK and LC3B protein content in LPS group increased significantly , also the level of IL -1 beta and IL -6 showed an increase (P<0.05).Compared with LPS group, the level of AMPK and LC3B increased significantly, however the level of IL -1βand IL -6 showed a decrease in APN group (P<0.05). Compared with APN group , the level of AMPK and LC3B were decreased , and level of IL-1βand IL-6 showed an increase in D group .Conclusion Adiponectin may regulate the inflammatory response mediated by LPS , and its mechanism may be related to the activation of AMPK pathway and the enhancement of autophagy .