CAJ | 학술논문

目的探讨骨髓间充质干细胞(BMSCs)对脓毒症小鼠急性肺损伤(ALI)的治疗作用及机制. 方法采用盲肠结扎穿孔术(CLP)复制小鼠脓毒症ALI模型,125只Balb/c小鼠随机分为五组(每组25只):假手术组( C组)、三个不同剂量BMSCs治疗组( L、M和H组)及脓毒症组(CLP组). 其中15只小鼠用于观察存活率,剩余10只用于观察其他指标. 取第三代BMSCs,在制模成功5 min后经小鼠尾静脉注射入小鼠体内,L、M和H组分别注入BMSCs 1 ×10 4个、1 ×10 5个和1 ×106个,C组及CLP组注射等量生理盐水. 在给予BMSCs后24 h留取标本,检测肺组织形态学、肺组织湿干质量比( W/D)、动脉血氧分压( PaO2 )和二氧化碳分压( PaCO2 ) ,血乳酸、血清TNF-α、IL-10及可溶性E选择素水平. 结果与CLP组比较,H组24 h及48 h存活率提高, 24 h肺微血管充血、出血及炎症细胞浸润减少,病理评分、W/D值、PaCO2、血乳酸、TNF-α、可溶性E选择素水平降低,PaO2及IL-10水平增加,差异均有统计学意义( P<0.05). 结论 BMSCs可抑制Balb/c小鼠ALI时血清TNF-α、可溶性E选择素水平,上调IL-10水平,减轻肺损伤严重程度,且该保护作用与BMSCs的剂量有关.
목적 탐토골수간충질간세포(BMSCs)대농독증소서급성폐손상(ALI)적치료작용급궤제. 방법 채용맹장결찰천공술(CLP)복제소서농독증ALI모형,125지Balb/c소서수궤분위오조(매조25지):가수술조( C조)、삼개불동제량BMSCs치료조( L、M화H조)급농독증조(CLP조). 기중15지소서용우관찰존활솔,잉여10지용우관찰기타지표. 취제삼대BMSCs,재제모성공5 min후경소서미정맥주사입소서체내,L、M화H조분별주입BMSCs 1 ×10 4개、1 ×10 5개화1 ×106개,C조급CLP조주사등량생리염수. 재급여BMSCs후24 h류취표본,검측폐조직형태학、폐조직습간질량비( W/D)、동맥혈양분압( PaO2 )화이양화탄분압( PaCO2 ) ,혈유산、혈청TNF-α、IL-10급가용성E선택소수평. 결과 여CLP조비교,H조24 h급48 h존활솔제고, 24 h폐미혈관충혈、출혈급염증세포침윤감소,병리평분、W/D치、PaCO2、혈유산、TNF-α、가용성E선택소수평강저,PaO2급IL-10수평증가,차이균유통계학의의( P<0.05). 결론 BMSCs가억제Balb/c소서ALI시혈청TNF-α、가용성E선택소수평,상조IL-10수평,감경폐손상엄중정도,차해보호작용여BMSCs적제량유관.
Objective To explore the possible mechanism and protective effect of marrow derived mesenchymal stem cells (BMSCs) on sepsis mice with acute lung injury (ALI).Methods Sepsis mice with ALI were induced by cecal ligation and puncture (CLP).One hundred and twenty -five Balb/c mice were randomly divided into five groups , twenty -five mice for every group .Sham control group (C group), BMSCs treatment groups (L, M, H group) and ALI group (CLP group). Five minutes after CLP, the mice in BMSCs treatment groups were intravenous administrated BMSCs though caudal vein of the mice (1 ×104 for L group, 1 ×105 for M group, 1 ×106 for H group), the other two groups were given normal saline of the same quantity .Fifteen mice in each group were used to observe the survival status and the others were used to collect samples for assessment of histology , lung wet to dry ratio (W/D) arterial partial pressure of oxygen and carbon dioxide (PaO2 and PaCO2), lactic acid, level of serum TNF-α, IL-10 and soluble E selectin .Results Compared with CLP group , the survival rate in H group at 24 h and 48 h were significantly improved .What's more, there was a significant decrease in W/D, lung injury score, PaCO2, level of lactic acid, serum TNF-αand souble E selectin, a significant increase in PaO2 and level of serum IL -10, in H group mice compared with CLP group mice at 24 h.Conclusion Treatment with BMSCs significantly reduces CLP -induced acute lung injury in Balb/c mice by inhibiting serum level of TNF -α, souble E selectin and increasing level of serum IL-10, and the effect is related to its dose .