CAJ | 학술논문

目的：探讨聚维酮碘( PVP-I )作为脱钙骨基质( demineralized bone matrix，DBM )长期保存剂的可行性。方法选用4种标准菌种进行 PVP-I 重复5次的灭菌效果验证。分别采用 CCK-8法、PNPP 法、RT-PCR 法检测 PVP-I 对成骨细胞的增殖、前交叉韧带( anterior cruciate ligament，ACL )活力以及成骨分化标志分子 COL1α、ALP、RUNX2、OCN mRNA 表达的影响；裸小鼠肌间隙植入实验验证 PVP-I 长期保存的 DBM 成骨活性是否受到影响。结果 PVP-I 对金黄色葡萄球菌、白色念珠菌、枯草杆菌黑色变种芽孢的杀灭率均为100%，对大肠杆菌的杀灭率为88.7%；与对照组(1±0.09)相比，高浓度 PVP-I 抑制细胞生长(0.63±0.03)(P＜0.05)，低浓度 PVP-I 不促进也不抑制细胞增殖；PVP-I 显著增强成骨细胞的 ALP 活力，上调 COL1α、ALP、RUNX2 mRNA 的表达量(1.25±0.15、1.38±0.14、1.23±0.09)(P＜0.05)，对 OCN mRNA 的表达影响不明显；裸鼠肌间隙植入实验表明，PVP-I 长期保存的 DBM 其成骨活性不受影响。结论 PVP-I 有望被开发成 DBM 的保存剂。
목적：탐토취유동전( PVP-I )작위탈개골기질( demineralized bone matrix，DBM )장기보존제적가행성。방법선용4충표준균충진행 PVP-I 중복5차적멸균효과험증。분별채용 CCK-8법、PNPP 법、RT-PCR 법검측 PVP-I 대성골세포적증식、전교차인대( anterior cruciate ligament，ACL )활력이급성골분화표지분자 COL1α、ALP、RUNX2、OCN mRNA 표체적영향；라소서기간극식입실험험증 PVP-I 장기보존적 DBM 성골활성시부수도영향。결과 PVP-I 대금황색포도구균、백색념주균、고초간균흑색변충아포적살멸솔균위100%，대대장간균적살멸솔위88.7%；여대조조(1±0.09)상비，고농도 PVP-I 억제세포생장(0.63±0.03)(P＜0.05)，저농도 PVP-I 불촉진야불억제세포증식；PVP-I 현저증강성골세포적 ALP 활력，상조 COL1α、ALP、RUNX2 mRNA 적표체량(1.25±0.15、1.38±0.14、1.23±0.09)(P＜0.05)，대 OCN mRNA 적표체영향불명현；라서기간극식입실험표명，PVP-I 장기보존적 DBM 기성골활성불수영향。결론 PVP-I 유망피개발성 DBM 적보존제。
Objective To discuss the feasibility of PVP-I being the preservation of demineralized bone matrix ( DBM ).Methods Four standard strains were used to perform the sterilization experiment in repeated 4 times. PVP-I was added to MC3T3-E1, then cell proliferation and viability were determined by CCK-8 and differentiation status by PNPP and RT-PCR. Muscle implant experiment of nude mice was performed to verify whether the activity of DBM was damaged since it was immersed in PVP-I for a long time.Results The sterilization rates of PVP-I to staphylococcus aureus, monilia albican and bacillus subtilis were all of 100%, while to Escherichia coli 88.7%. CCK-8 assay showed reduced proliferation of the MC3T3-E1 cells when the PVP-I was at a high concentration ( 0.63±0.03 ) (P<0.05 ). When the concentration of PVP-I was down-regulated, a negligible effect was observed on MC3T3-E1 growth. PVP-I signiifcantly enhanced the activity of ALP and up-regulated the expressions of COL1α, ALP and RUNX2 ( 1.25±0.15, 1.38±0.14, 1.23±0.09 ) (P<0.05 ). However, PVP-I did not affect the expression of OCN. The nude mice experiment suggested that the activity of DBM was not inlfuenced by PVP-I.Conclusions PVP-I may be applied as the preservation of DBM.