中国动物传染病学报
中國動物傳染病學報
중국동물전염병학보
Chinese Journal of Veterinary Parasitology
2015年
4期
31-36
,共6页
王少辉%刘萍萍%魏建超%邵东华%史子学%李蓓蓓%马志永
王少輝%劉萍萍%魏建超%邵東華%史子學%李蓓蓓%馬誌永
왕소휘%류평평%위건초%소동화%사자학%리배배%마지영
单增李斯特菌%耐药性%血清型%生物被膜
單增李斯特菌%耐藥性%血清型%生物被膜
단증리사특균%내약성%혈청형%생물피막
Listeria monocytogenes%resistance antibiotics%serotype%biofilm
为了解上海市动物源性食品中单增李斯特菌的污染状况,在上海市不同超市和农贸市场采集479份动物源性食品样品,依据国标方法进行菌株分离,并对分离菌株的耐药性、血清型及生物被膜形成能力进行测定。结果共分离鉴定34株单增李斯特菌,分离率为7.1%(34/479)。药敏试验结果显示单增李斯特菌分离株的耐药率虽然不高,但日趋严重,对氨苄青霉素和万古霉素均敏感,对头孢曲松的耐药性最高(55.88%),林可霉素次之(41.18%)。血清型鉴定表明单增李斯特菌分离株以血清型1/2a(3a)型为主(76.47%),血清型1/2c(3c)次之(17.65%),而致病性强的血清型4b(4d、4e)仅占2.94%。生物被膜形成能力实验表明,所有的菌株均能形成生物被膜,其中76.47%(26/34)分离株生物被膜形成能力微弱。上海市动物源性食品中单增李斯特菌污染情况较为严重,主要流行1/2a(3a)血清型,耐药率日趋严重,均可形成生物被膜。因此,应加强对动物源性食品中单增李斯特菌的监控。
為瞭解上海市動物源性食品中單增李斯特菌的汙染狀況,在上海市不同超市和農貿市場採集479份動物源性食品樣品,依據國標方法進行菌株分離,併對分離菌株的耐藥性、血清型及生物被膜形成能力進行測定。結果共分離鑒定34株單增李斯特菌,分離率為7.1%(34/479)。藥敏試驗結果顯示單增李斯特菌分離株的耐藥率雖然不高,但日趨嚴重,對氨芐青黴素和萬古黴素均敏感,對頭孢麯鬆的耐藥性最高(55.88%),林可黴素次之(41.18%)。血清型鑒定錶明單增李斯特菌分離株以血清型1/2a(3a)型為主(76.47%),血清型1/2c(3c)次之(17.65%),而緻病性彊的血清型4b(4d、4e)僅佔2.94%。生物被膜形成能力實驗錶明,所有的菌株均能形成生物被膜,其中76.47%(26/34)分離株生物被膜形成能力微弱。上海市動物源性食品中單增李斯特菌汙染情況較為嚴重,主要流行1/2a(3a)血清型,耐藥率日趨嚴重,均可形成生物被膜。因此,應加彊對動物源性食品中單增李斯特菌的鑑控。
위료해상해시동물원성식품중단증리사특균적오염상황,재상해시불동초시화농무시장채집479빈동물원성식품양품,의거국표방법진행균주분리,병대분리균주적내약성、혈청형급생물피막형성능력진행측정。결과공분리감정34주단증리사특균,분리솔위7.1%(34/479)。약민시험결과현시단증리사특균분리주적내약솔수연불고,단일추엄중,대안변청매소화만고매소균민감,대두포곡송적내약성최고(55.88%),림가매소차지(41.18%)。혈청형감정표명단증리사특균분리주이혈청형1/2a(3a)형위주(76.47%),혈청형1/2c(3c)차지(17.65%),이치병성강적혈청형4b(4d、4e)부점2.94%。생물피막형성능력실험표명,소유적균주균능형성생물피막,기중76.47%(26/34)분리주생물피막형성능력미약。상해시동물원성식품중단증리사특균오염정황교위엄중,주요류행1/2a(3a)혈청형,내약솔일추엄중,균가형성생물피막。인차,응가강대동물원성식품중단증리사특균적감공。
This study was performed to investigate the contamination ofListeria monocytogenes (LM) in animal origin food in Shang-hai, which gained further insights on the public health risk caused by this food borne pathogen. A total of 479 animal origin food samples were screened forLM. Then, the antibiotic sensitivity, serotype and biofilm formation of each isolate were determined. The results showed that 34LM strains were isolated from 479 samples, which accounted for 7.1%. The antibiotics resistance tests showed that resistant rate ofLM isolates were not high. AllLM isolates were sensitive to ampicillin and vancomycin. However, 55.88% isolates were resistant rate to ceftriaxone, followed by lincomycin (41.18%). Serotying results showed that 76.47%LM strains were belonged to serovar 1/2a(3a), followed by serovar 1/2c(3c). Whereas, only one isolate (2.94%) was the pathogenic serotype 4b(4d、4e). Bioilm formation assays showed that all theLM isolates could produce biofilm, in which 76.47%(26/34)were weak biofilm producers. These results indicated that animal origin food was contatiminated byLM, and the biofilm will increase the chances ofLM contamina-tion. Thus, it is necessary to inspect and survey the contamination byLM on farms and in abattoirs to reduce the incidence of foodborne infections in humans.