CAJ | 학술논문

本实验探究了不同浓度的石油污染物对涡虫体内过氧化氢酶（ CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH - PX）三种酶活性的影响.通过急性毒理实验，研究了石油污染物对涡虫毒理作用的24 h半数致死浓度（LD50）为7.209 g/ L.在安全浓度条件下，选取0、0.5、1、1.5、2、2.5、3 g/ L 七个浓度进行处理，处理时间为24 h.结果表明：石油污染物对涡虫体内3种抗氧化酶活力均有明显影响，随着石油污染物浓度的升高，CAT酶活性总体呈下降趋势；SOD 酶活性总体呈缓慢下降趋势；GSH - PX 酶活性总体呈明显下降趋势.实验说明涡虫对石油污染物非常敏感，可以为石油污染检测等提供理论依据.
본실험탐구료불동농도적석유오염물대와충체내과양화경매（ CAT）、초양화물기화매（SOD）、곡광감태과양화물매（GSH - PX）삼충매활성적영향.통과급성독리실험，연구료석유오염물대와충독리작용적24 h반수치사농도（LD50）위7.209 g/ L.재안전농도조건하，선취0、0.5、1、1.5、2、2.5、3 g/ L 칠개농도진행처리，처리시간위24 h.결과표명：석유오염물대와충체내3충항양화매활력균유명현영향，수착석유오염물농도적승고，CAT매활성총체정하강추세；SOD 매활성총체정완만하강추세；GSH - PX 매활성총체정명현하강추세.실험설명와충대석유오염물비상민감，가이위석유오염검측등제공이론의거.
The impact of petro - pollutants on activities of Dugesia japonicaˊs catalase( CAT),superoxide dismutase(SOD),glutathione peroxidase(GSH - PX)were studied,and the results showed that the lethal con-centration 50(LD50 )of petro - pollutants on Dugesia japonica at 24h was 7. 209g / L. In the safe concentration range,Dugesia japonica was trained for 24 hours with petro - pollutants solutions of 0,0. 5,1,1. 5,2,2. 5,3g /L respectively. The results showed that the petro - pollutants had a significant effect on the activities of the en-zyme. With the increase of petro - pollutants concentration,the CAT enzyme activity is a downward trend in gen-eral;the SOD enzyme activity showed a trend of slow downtrend;and the GSH - PX activity appeared a dramatic downward trend. Itˊs concluded that Dugesia japonica is very sensitive and will provide the theoretical basis for the detection of petro - pollutants.