CAJ | 학술논문

选择小鼠脾脏和外周血T淋巴细胞作为研究对象，通过体外试验探讨黄芪多糖的免疫调节作用。用不同剂量的黄芪多糖作用于红细胞和 T 淋巴细胞共同培养体系，48 h 后，应用 MTT 比色法检测细胞的增殖活性，研究黄芪多糖对红细胞免疫调控功能的影响。结果表明，小鼠红细胞、植物血凝素（PHA），单独作用对 T 淋巴细胞的增殖均有显著的促进作用，且红细胞对 PHA 激活的 T 淋巴细胞的增殖也有促进作用，但是二者没有明显的协同促进作用；黄芪多糖不能促进体外培养 T 淋巴细胞增殖，甚至还抑制 PHA诱导的 T 淋巴细胞的增殖活性；但黄芪多糖可以协助红细胞对淋巴细胞的免疫调控功能，双方共同作用，引起淋巴细胞数目的增加和增强增殖功能。
선택소서비장화외주혈T림파세포작위연구대상，통과체외시험탐토황기다당적면역조절작용。용불동제량적황기다당작용우홍세포화 T 림파세포공동배양체계，48 h 후，응용 MTT 비색법검측세포적증식활성，연구황기다당대홍세포면역조공공능적영향。결과표명，소서홍세포、식물혈응소（PHA），단독작용대 T 림파세포적증식균유현저적촉진작용，차홍세포대 PHA 격활적 T 림파세포적증식야유촉진작용，단시이자몰유명현적협동촉진작용；황기다당불능촉진체외배양 T 림파세포증식，심지환억제 PHA유도적 T 림파세포적증식활성；단황기다당가이협조홍세포대림파세포적면역조공공능，쌍방공동작용，인기림파세포수목적증가화증강증식공능。
In this study,T-lymphocytes from mouse spleen and peripheral blood were used for in vitro ex-periments to explore the immunoregulatory effects of Astragalus polysaccharide (APS).Co-cultures of red blood cells and T-lymphocytes were treated with different doses of APS for 48 h.MTT colorimetry was then used to determine cell proliferation in order to assess the effect of APS on the immunoregulatory func-tion of red blood cells.Both mouse red blood cells and PHA significantly promoted T-lymphocyte prolifer-ation when used alone.Red blood cells also promoted the proliferation of PHA-activated T-lymphocytes. However,the two did not show any significant synergistic effect.APS did not promote the proliferation of T-lymphocytes cultured in vitro and even inhibited the proliferation of PHA-activated T-lymphocytes. However,APS could aid the immunoregulatory effect of red blood cells on lymphocytes,and the combined effect of the two would enhance lymphocyte proliferation markedly.